L-精氨酸对大鼠局灶性脑缺血再灌注早期炎症损伤研究

目的 探讨一氧化氮（NO）前体L-精氨酸（L-Arg）对大鼠局灶性脑缺血再灌注早期炎症损伤的影响.方法 建立大鼠局灶性脑缺血再灌注模型,设立假手术组、缺血组（ischemia/reperfusion,IS组）,L-Arg治疗组（n=10）.L-Arg治疗组于缺血2h再灌注即刻给予L-Arg,缺血组给予生理盐水.将大鼠于缺血再灌注6h后断头取脑,检测脑组织中肿瘤坏死因子（TNF-α）、白细胞介素6（IL-6）和白细胞介素10（IL-10）的活性,测定脑组织含水量和脑梗死体积.结果 局灶性脑缺血再灌注大鼠给予L-Arg治疗组TNF-α和IL-6的表达下降,IL-10升高,脑组织含水量及脑梗死面积减少.结论 L-精氨酸可以通过抑制脑组织炎性因子TNF-α和IL-6的表达,上调IL-10的含量来发挥抗炎作用,从而对大鼠局灶性脑缺血再灌注损伤早期产生一定程度的保护作用.

The Influence of L-Arginine on Early Reperfusion Injury of Focal Cerebral Ischemia in Rats

Objective To explore the role of L-Arginine （L-Arg） one of NO precursor on early stage of focal cerebral isehemia-reperfusion inflammatory injury in rats. Methods The rat model of focal cerebral ischemia injury were established and divided into sham operation group, isehemia reperfusion group and L-Arg treatment group（10 rats in each group）. L - Arg was given immediately after 2 hours of cerebral ischemia in L-Arg treatment group, saline solution was given in ischemia group. The rats of each group were beheaded after 6 hours focal cerebral ischemia-reperfusion. The levels of inflammatory cytokines tumor necrosis factor（TNF-α） , interleukin 6（IL-6） and interleukin 10（IL-10） in beheaded brain of rats were detected. The brain infarction area and water content in brain tissue were also determined. Results The levels of inflammatory factor TNF-α and IL-6 in L-Arg treatment group rats were reduced and the expression of IL-10 was raised, the brain tissue water content and cerebral infarction area were also reduced. Conclusion L-Arginine play a role of anti- inflammatory by inhibiting the brain tissue inflammatory factor TNF and IL-6 and increasing the content of IL- 10, which produces a degree of protection on cerebral ischemia in rats.