HCG稀释检测对结果的影响及解决策略探讨

目的 研究HCG稀释检测对结果的影响,继而优化检测流程,指导得出准确的检测结果.方法 随机选取需进行HCG检测的患者标本35份,使用罗氏Cobas e601电化学发光仪检测标本在原倍和50倍稀释条件下HCG数值,比较检测结果间的差别.结果 在一定的数值范围（1770 mIU/mL）内,稀释后结果要低于原倍结果,而超出这个范围稀释后结果要高于原倍结果,可以其为切割值对稀释检测的结果应按照Y=1.020X＋ 34.99和Y=0.877X＋ 228.2进行校正.结论 HCG的检测应根据临床诊断决定检测模式,并将检测结果进行校正,确保检测结果的准确可靠.

Influence of Sample Dilution on Detection Results of HCG

Objective To investigate the influence of HCG sample dilution on its detection results and to optimize the detection flow sheet and acquire accurate test results. Methods The 35 blood samples from patients were randomly collected and the HCG concentration in original samples or diluted with 50 times solution samples were detected by chemiluminescence immunoassay with Roche cobas e601. Results At the HCG levels was lower than 1770 mIU/mL, the HCG concentration detected by diluted method was lower than that of original samples. On the other hand, the HCG detected results with dilution method was higher than original samples detection. The detected results with dilution method needed to rectify by the formulas. Conclusion In order to ensure the accuracy of the HCG detection result, the appropriate detection mode should be chosen and the result should be rectified based on the detection model.