血清基质金属蛋白酶-3与类风湿关节炎的相关性研究

目的 探讨血清基质金属蛋白酶-3（matrix metalloproteinases-3,MMP-3）与类风湿关节炎（rheumatoid arthritis,RA）及其炎症反应的相关性.方法 用免疫比浊法（immunoturbidimetric assays）检测146例RA患者（RA组）及232例非RA人群（对照组）血清MMP-3水平;对两组MMP-3结果进行ROC曲线分析;分析RA患者血清MMP-3与反映RA患者炎症活动程度临床指标的关系.结果 RA组血清MMP-3浓度为170.00±19.36ng/mL,明显高于对照组的47.92±1.98ng/mL,差异有统计学意义（P＜0.001）.血清MMP-3诊断RA的ROC曲线下面积为0.731,取诊断界限值为71.65 ng/mL时,敏感度为58.9％,特异度为88.79％.RA组患者血清MMP-3水平与其他RA诊断以及反应RA炎症反应的指标,如C反应蛋白（C-reaction protein,CRP）、前白蛋白（prealbumin,PA）、血沉（erythrocyte sedimentation rate,ESR）、血小板（platelet）以及白细胞计数（WBC）均具有显著相关性,P值均＜0.001.结论 RA患者血清MMP-3水平显著高于正常入,其水平与反映RA患者炎症活动程度临床指标CRP、PA、ESR、PLT以及WBC具有显著相关性.类风湿性关节炎患者血清MMP-3水平与类风湿性关节炎的炎症活动性有关.

Correlation of Matrix Metalloproteinases-3 and Rheumatoid Arthritis

Objective To investigate the relationship between serum matrix metalloproteinase-3 and rheumatoid arthritis. Methods The serum MMP-3 level in 146 patients with RA and 232 non RA patients were detected by immunoturbidimetry. The receiver operating Curve （ROC） analysis was carried out to get the cut- off value. The relationship between MMP-3 and other clinical biochemical indices were analyzed by multiple regressions. Results The serum MMP-3 concentration in RA group（ 170 ±19.36 ng/mL） was significantly higher than that of control group（47.92 ± 1.98 ng/mL,P 〈0.001 ）. The optimal diagnostic cut-off value for MMP-3 was 71.65 ng/mL. The sensitivity and specificity were 58.9% and 88.79% respectively at this cut-off value. The serum concentration of MMP-3 in RA patients was significantly correlated with inflammation and activity indices such as C-reaction protein, prealbumin, erythrocyte sedimentation rate, platelet, white blood cell count（ P 〈 0. 001 ）. Conclusion The serum MMP-3 levels in patients with rheumatoid arthritis is correlated to inflammatory activity.