孕妇血清及其胎儿脐血HBV-DNA定量检测与宫内感染的关系

目的：探讨新生儿发生宫内感染与孕妇不同的乙肝感染状态的关系。方法：通过ELISA法及荧光定量PCR法检测血清标本中HBV标志物和HBV-DNA含量水平。结果：乙肝大三阳孕妇其体内HBV-DNA水平明显高于乙肝小三阳孕妇，胎儿脐血HBV-DNA水平与孕妇血清中HBV-DNA水平密切相关，孕妇血HBV-DNA含量高时脐血HBV-DNA阳性率明显增高。结论：宫内感染的发生率与孕妇血清HBV-DNA含量相关，脐血HBV-DNA阳性是判断HBV宫内感染的相关指标。

The Relationship Between Detection of HBV-DNA in Pregnant Women or Cord Blood and Intranternine Infection of Fetus

Objective To study the relationship of HBV infections status in pregnant women to intranterine fetal infective. Methods HBV markers and HBV-DNA level in the serum were detected by ELISA and fluorescent quanfitated PCR. Results HBV-DNA in group of HBsAg, HbeAg, HbcAb was significantty higher than the group of HBsAg, HbeAb, HbcAb,Cord blood HBV-DNA level was correlated to serous HBV-DNA content. Conclusion Incidence of intrauterine HBV infections is correlated to serous HBV-DNA level and highly correlated to viremia in advanced stage pregnant, the intrauterine infection is associated with high level HBV-DNA of cord blood which is one of the important factors.