大鼠脑缺血再灌注后脑皮质电图及海马电图功率值的动态变化及中药9602的影响

目的:观察选择性易损脑区缺血后皮层电图(ECoG)和海马电图(EHG)相对功率随时间的动态变化及中药9602的影响。方法:采用慢性埋植皮层及海马电极的方法,研究大鼠脑缺血再灌注后ECoG与EHG的变化过程。结果:大鼠ECoG额区各频段及总功率8h时普遍低于假手术组,24h时回升,72h后逐渐降低;枕区在72h时θ频段开始下降;7d时各频段及总功率均明显低于假手术组。EHG的θ、α及总功率在脑缺血后8h、24h、72h均已明显低于假手术组;7d时各频段及总功率与假手术组差异非常显著。9602及阳性药组脑缺血后ECoG和EHG功率值均明显高于模型组。结论:脑缺血再灌注后ECoG、EHG功率逐渐下降,可能与脑缺血后DND的发生关系密切。9602明显阻止脑缺血后ECoG和EHG功率的下降,可能与其神经保护作用有关。

Dynamic changes in power of rat''s brain electrocorticogram and electrohippocampogram after cerebral ischemia and the effects of 9602

AIM and METHODS:To observe the dynamic changes in relative power of rats brain electrocorticogram(ECoG) and electrohippocampogram(EHG) after ischemia, we studied the changes of rat's ECoG and EHG after ischemia using the method of chronic implanting electrodes in cortex and hippocampus and the effects of Chinese herbs 9602 on them. RESULTS:The frontal ECoG relative power of all bands and total power decreased in 8 hours after ischemia, restored in 24 hours and decreased again after 72 hours. It decreased significantly as compared with that of sham-control 7 day postischemia. The occipital θ band at 72 hour, all bands and total power decreased 7 day after ischemia. As compared with sham-operated control, the θ、α and total power of EHG decreased notably at 8, 24 and 72 h after ischemia. The changes were more markedly in 7 day after ischemia. Treated with 9602 and hydergine, the ischemic animals showed significant elevation of ECoG and EHG relative power in comparison with ischemic rats. CONCLUSION:The power of ECoG and EHG decreased after ischemia, it may be correlated with delayed neuronal death induced by ischemia. 9602 prevented the decreases in ECoG and EHG power obviously after ischemia,which probably related to the neuroprotective effects.