| 紫外线诱导HaCat转铁蛋白受体表达机制的初步探讨 |
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| 引用本文： | 李燕华,毕志刚.紫外线诱导HaCat转铁蛋白受体表达机制的初步探讨[J].中国皮肤性病学杂志,2006,20(9):516-518. |
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| 作者姓名： | 李燕华 毕志刚 |
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| 作者单位： | 南京医科大学第一附属医院皮肤科,江苏,南京,210029 |
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| 基金项目： | 国家自然科学基金;江苏省重点学科建设项目 |
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| 摘 要： | 目的探讨紫外线诱导转铁蛋白受体(TFR)表达的机制。方法以紫外线(UVB)照射HaCat,流式细胞仪检测TFR的表达,realtime-PCR检测TFRmRNA表达。结果UVB可增强TFR的表达,并呈剂量依赖性和时间依赖性。10mJ/cm2的UVB就能促进TFR表达,在紫外线照射后6h开始表达。UVB以剂量依赖性方式诱导TFR的mRNA表达,在UVB照射后8h,TFRmRNA表达水平达高峰,16h开始下降。结论UVB可增强TFR的表达。
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| 关 键 词： | 紫外线 TFR HaCat |
| 文章编号： | 1001-7089（2006）09-0516-03 |
| 收稿时间： | 2006-03-10 |
| 修稿时间： | 2006-03-102006-04-05 |
Explore The Mechanism of UV-Induced TFR Expression in Hacat Cells |
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| LI Yan-hua,BI Zhi-gang.Explore The Mechanism of UV-Induced TFR Expression in Hacat Cells[J].The Chinese Journal of Dermatovenereology,2006,20(9):516-518. |
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| Authors: | LI Yan-hua BI Zhi-gang |
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| Institution: | Department of dermatology, the First Affiliated Hospital of Nanfing Medical University, Nanjing 210029, China |
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| Abstract: | Objectives To explore the mechanism of UV-induced TFR expression of HaCat. Methods HaCat cells were irradiated with UVB,Flow Cytometry detect TFR expression. The TFR mRNA expression was determined by reahime-PCR method. Results UVB was able to induce TFR expression in a dose- and time-dependent manner in HaCat cells. Compared with control groups, 10 mJ/cm^2 UVB can enhance the expression of TFR and begin to express at 6h after UVB irradiation. Realtime-PCR indicate that UVB also induce TFR mRNA expression in a dose-manner. With control groups,TFR mRNA expression reach a peak at 8 h in HaCat cells and begin to decreased at 16 h after UVB irradiation. Conclusion UVB can enhance the expression of TFR. |
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| Keywords: | TFR HaCat |
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