神经节苷酯对大鼠脑缺血神经细胞凋亡的影响

目的研究神经节苷脂(GM)对大鼠脑缺血神经细胞凋亡的抑制作用及可能机制。方法线栓法制作大鼠大脑中动脉栓塞模型,♂SD大鼠,随机分成假手术组、模型对照组、GM低、中、高剂量治疗组,缺血48 h后各组大鼠神经功能缺陷评分,测定脑组织一氧化氮(NO)的含量,检测脑细胞凋亡率,分析脑缺血区诱生型一氧化氮合酶(iNOS)和Caspase-3 mRNA的表达。结果与假手术组比较,模型对照组、GM低、中、高剂量治疗组神经功能缺陷评分、NO含量、凋亡率、iNOS和Caspase-3表达量均增加,差异有统计学意义(P<0.05);与模型对照组和GM低剂量治疗组比较,GM中、高剂量治疗组均明显下降,差异有统计学意义(P<0.05);模型对照组和GM低剂量治疗组之间差异无统计学意义(P>0.05),GM中剂量治疗组和高剂量治疗组之间差异也无统计学意义(P>0.05)。结论神经节苷酯减少iN-OS生成,抑制细胞凋亡,对缺血神经细胞产生保护作用。

Effect of ganglioside on neuron apoptosis after focal cerebral ischemia in rats

Aim To investigate the inhibition of neuron apoptosis after cerebral ischemia by gangliaside and its possible mechanism.Methods The model of middle cerebral artery occluasion was prepared by using a filament,and Male SD rats were randomly divided into sham-operated group,model group and gangliaside-treated groups,including low dose group,middle dose group and high dose group,respectively.The neurological function was scored.The content of NO was measured.And the percentage of neuron apoptosis was detected.The expressions of iNOS and Caspase-3 mRNA were analyzed.Results In comparison with the sham-operated group,the score of nerve function,NO content,apoptosis percentage,and iNOS and Caspase-3 mRNA increased in the model groups,the low dose group,the middle dose group and the high dose group with the statistical significance(P<0.05).In comparison with the model group and the low dose group,those decreased in the middle dose group and the high dose group had statistical significance(P<0.05).There was no statistical difference between the model group and the low dose group(P>0.05,as well as between the middle dose group and the high dose group(P>0.05).Conclusions In vivo ischemia models,the suitable dose GM can reduce the expression of iNOS,inhibit the cell apoptosis and protect the nerve cell.