介入治疗对兔VX2肝癌MMP-2基因表达的影响

目的研究MMP-2在兔VX2肝癌中表达的意义以及介入治疗对其表达的影响。方法建立兔VX2肝癌的动物模型,将实验动物分为介入组和对照组,比较2组的肝功能变化、肿瘤体积增长率,用原位杂交和免疫组化方法研究各组的MMP-2基因及蛋白表达情况。结果对照组的AST和ALT分别高于同一时间段介入组。各组肿瘤随着时间延长体积均增大,但介入治疗后尤其是术后2、3周体积增长减缓,与对照组相比,有显著性差异。原位杂交及免疫组化显示MMP-2mRNA及蛋白表达主要定位于癌细胞胞浆。介入组MMP-2mRNA及蛋白表达的阳性率较对照组明显减少(P<0·05)。转移组MMP-2mRNA及蛋白表达的阳性率较无转移组明显增加(P<0·05)。结论介入治疗能改善肝功能,延缓肿瘤生长,减少转移。MMP-2的高表达预示着高转移潜能。

Effect of Interventional Treatment on MMP-2 Gene Expression of Rabbit VX2 Liver Tumor

Objective To study the expression of matrix metalloprotein-2(MMP-2) and discuss the value of interventional treatment in rabbit VX_2 liver tumor.Methods 48 rabbit models were randomly divided into study group(n=24) and control group (n=24).The hepatic function,tumor size in two groups were compared.The level of MMP-2 was tested with in situ hybridization and immunohistochemical technology.Results MMP-2 was located in the cytoplasm of tumor cells.The expression of MMP-2 mRNA and protein in control group was significantly lower than that of the study group (P<0.05).In the metastasis group,the level of MMP-2 mRNA and protein was markedly higher than that of no metastasis group(P<0.05).Conclusion The growth of rabbit liver tumors and metastasis could partly be inhibited by interventional treatment.The high expression of MMP-2 indicates the trend of high metastasis.