| 补骨脂素逆转多药耐药细胞系K562/ADR耐药性研究 |
| |
| 引用本文： | 蔡宇,蔡天革. 补骨脂素逆转多药耐药细胞系K562/ADR耐药性研究[J]. 中国药理学通报, 2003, 19(10): 1164-1166 |
| |
| 作者姓名： | 蔡宇 蔡天革 |
| |
| 作者单位： | 1. 暨南大学药学院,广州,510632
2. 辽宁大学环境与生命科学学院,沈阳,110036 |
| |
| 基金项目： | 国家自然科学基金资助课题,No 3 0 160 0 98，浙江省教育厅科研计划项目资助,No 2 0 0 2 0 73 6 |
| |
| 摘 要： | 目的 研究补骨脂素对白血病细胞阿霉素耐药株(K5 6 2 /ADR)多药耐药 (multidrugresistance,MDR)性的逆转作用及其机制。方法 采用MTT法检测药物细胞毒性作用 ,高效液相色谱法检测细胞内阿霉素 (ADR)的浓度 ,流式细胞术测定细胞P 糖蛋白 (P gp)的表达。 结果 补骨脂素 (1～ 2 0 μmol·L-1)能不同程度地降低ADR对K5 6 2 /ADR细胞的IC50 。 2 0 μmol·L-1能显著提高ADR在K5 6 2 /ADR细胞内的浓度 ,降低K5 6 2 /ADR细胞P gp的表达。 结论 补骨脂素能逆转K5 6 2 /ADR细胞的MDR ,其机制与抑制P gp的功能及其表达 ,增加细胞内ADR的积累有关
|
| 关 键 词： | 补骨脂素 多药耐药 K562/ADR 逆转 |
| 文章编号： | 1001-1978(2003)10-1164-03 |
| 修稿时间： | 2003-03-28 |
Reversal effect of psoralen on multidrug resistance of K562/ADR cells |
| |
| CAI Yu,CAI Tian Ge. Reversal effect of psoralen on multidrug resistance of K562/ADR cells[J]. Chinese Pharmacological Bulletin, 2003, 19(10): 1164-1166 |
| |
| Authors: | CAI Yu CAI Tian Ge |
| |
| Affiliation: | CAI Yu,CAI Tian Ge 1 |
| |
| Abstract: | AIM To research the reversal effect of Psoralen on K562/ADR and its mechanisms of reversing multidrug resistance (MDR). METHODS The cytotoxic effect of chemotherapeutic agents was determined by MTT assay. The intracellular adriamycin(ADR) concentration was assayed by HPLC. RESULTS Psoralen can reduce the value of IC 50 of ADR and against MDR cells line K562/ADR at non cytotoxic concentration and enhance accumulation of adriamycin. CONCLUSION Psoralen can reverse MDR of K562/ADR cells. Its reversal mechanisms were associated with the increase of the intracellular accumulation of ADR. |
| |
| Keywords: | psoralen reverse multidrug resistance K562/ADR cells |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
