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| 补肾中药HU-ECS对培养成骨细胞增殖、分化及矿化功能的影响 | |
| 引用本文： | 金慰芳,朱文箐,王洪复,骆宏丰,胡昌奇.补肾中药HU-ECS对培养成骨细胞增殖、分化及矿化功能的影响[J].中国骨质疏松杂志,2001,7(1):9-11. |
| 作者姓名： | 金慰芳 朱文箐 王洪复 骆宏丰 胡昌奇 |
| 作者单位： | 1. 上海医科大学老年医学研究中心 2. 上海医 科大学药学院天然药化教研室 3. 上海医科大学药学院天然药化教研室 |
| 基金项目： | 国家自然科学基金资助项目(39570774)；上海市教委重点学科资助项目 |
| 摘 要： | 目的：为了解补肾中药HU-ECS对体外培养成骨细胞的作用。方法：应用MTT法、对硝基苯磷酸盐法及茜素红染色方法观察HU-ECS对体外培养民骨细胞的增殖、ALP表达及矿化结节形成的影响。结果：HU-ECS具有刺激骨细胞增殖作用（10^-6g/ml组，P＜0.01-0.001），提高ALP活性及矿化结节形成的数量。结论：HU-ECS具有刺激体外培养成骨细胞增殖及分化成熟的功能。 |
| 关 键 词： | 补肾中药 细胞培养 成骨细胞 药理 |
Effects of HU-ECS on proliferation,differentiation and minera lization of cultured osteoblasts in vitro |
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| JIN Weifang,ZHU Wenjing,WANG Hongfu,et al..Effects of HU-ECS on proliferation,differentiation and minera lization of cultured osteoblasts in vitro[J].Chinese Journal of Osteoporosis,2001,7(1):9-11. | |
| Authors: | JIN Weifang ZHU Wenjing WANG Hongfu |
| Institution: | JIN Weifang,ZHU Wenjing,WANG Hongfu,et al.Research Center of Geriatric Medicine,Shanghai Medical University,Shanghai\ 200032,China\, |
| Abstract: | Objective\ To study the effects of HU ECS extracted from traditional Chinese medicine on cultured osteoblasts in vitro. Methods\ MTT,PNPP and ARS were used to observe the proliferation,activity of ALP and the number of mineral nodes of osteoblasts cultured in vitro. Results\ It was found that HU ECS had the effects on stimulating the proliferation,ALP activity and the number of mineral nodes of cultured osteoblasts In 10 -6 g/ml concentration,the effect was stable and notable. Conclusion\ HU ECS has the effects on stimulating the proliferation,differentiation and maturation of cultured osteoblasts in vitro. |
| Keywords: | Chinese drage \ Cultured cell \ Osteoblast |
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