岩黄连总碱抑制Tca8113细胞及其端粒酶活性的实验研究

目的:研究中草药岩黄连总碱(yanhuanglian total alkalions,YHL-TA)对人舌鳞状细胞癌Tca8113细胞增殖和端粒酶活性的影响及其抗癌机制。方法:采用噻唑蓝比色法(MTT法)检测YHL-TA浓度分别为0.200、0.100、0.050、0.025g/L和空白对照组Tca-8113细胞增殖的情况,同时用以PCR为基础的TRAP-ELISA法检测Tca8113细胞端粒酶活性的改变。所有数据采用SPSS13.0进行统计学分析。结果:岩黄连总碱能明显抑制Tca8113细胞增殖,且呈浓度和时间依赖性。72h时,0.200、0.100、0.050、0.025g/L岩黄连总碱组细胞增殖抑制率分别为(66.0±4.0)%、(57.5±3.2)%、(45.3±3.3)%、(37.9±5.5)%。72h时,0.100、0.050g/L组端粒酶活性低于空白对照组(P<0.05)。结论:岩黄连总碱可以明显抑制Tca8113细胞株的增殖及其端粒酶活性,抑制端粒酶活性可能是岩黄连总碱抗舌癌的机制之一。

Inhibition of Yanhuanglian Total Alkalions on Cell Proliferation and Telomerase Activity of Tca8113 Cell Lines

Objective: To investigate the effects of yanhuanglian total alkalions (YHL-TA) on cell proliferation and human telomerase activity of human Tca8113 cell lines. Methods: Tca8113 cell lines were treated by YHL-TA in concentration of 0.025?0.050?0.100?0.200 g/L respectively and compared with blank control groups. Cell proliferation inhibition ratio was assayed with MTT, and telomerase activity was detected by telomeric repeat amplification protocal enzyme linked immunosorbent assay based on PCR(TRAP-ELISA assay). Data were analyzed by one-way ANOVA. Results: Cell proliferation of Tca8113 were significantly inhibited by YHL-TA. The proliferation inhibiting rate of YHL-TA in concentration of 0.200,0.100, 0.050, 0.025 g/L on Tca8113 cell at 72 hours were (66.0±4.0)%, (57.5±3.2)%, (45.3±3.3)%, (37.9±5.5)% respectively(F=158.108,P<0.05). Result of ELISA assay showed that telomerase activity was decreased after treated by YHL-TA for 72 hours. Conclusion: YHL-TA shows the capability to inhibit the proliferation of Tca8113 cells and telomerase activity of Tca8113 cell lines. This may be one of the mechanisms for anticancer function of YHL-TA.