可移植性BALB/c小鼠红白血病模型的建立及其生物学特性

目的：利用７，１２二甲基苯蒽诱发的ＢＡＬＢ／ｃ小鼠红白血病，通过在同基因小鼠中连续移植建立一株可移植性ＢＡＬＢ／ｃ小鼠红白血病模型，并对其主要生物学特性进行研究。方法：用红白血病小鼠脾细胞给同基因型小鼠连续传代，经２０代的连续传代，发病率１００％，无自发缓解。结果：静脉接种的瘤细胞数量与存活时间呈线性关系，接种１０６白血病细胞平均生存期为１０．２±１．３天；瘤细胞主要侵及骨髓、脾及肝脏，一般不累及淋巴结和胸腺；晚期外周血出现大量瘤细胞，以早幼和中幼红细胞为主，晚期网织红细胞和血小板减少；血红蛋白染色呈阳性或弱阳性，过氧化物酶反应阴性，Ｔｈｙ１抗原、Ｆｃ受体阴性；电镜观察未见病毒样颗粒；对临床常用化疗药物反应敏感。结论：可移植性ＢＡＬＢ／ｃ小鼠红白血病模型的建立为研究红系增生和分化、非病毒性红系恶性疾病的发病学和实验治疗等提供了较为理想的动物模型。

Establishment and biological characterization of a transplantable BALB/c mouse erythroblastic leukemia model

OBJECTIVE: Biological characterization of a BALB/c mouse transplantable erythroblastic leukemia model EL9611. METHODS AND RESULTS: The original erythroblastic leukemia (EL) mouse was obtained by 7,12-DMBA induction. The spleen cell suspension from the original EL mouse was injected into the same inbred BALB/c strain mice. After successive transplantation for 20 generations, the incidence of EL was 100% without spontaneous remission. There was a linear relationship between the survival time of the EL mice and the number of leukemic cells inoculated. When 10(6) leukemic cells were inoculated intravenously, the EL mice survived 10.2 +/- 1.3 days. Invasion of leukemic cells involved mainly in the bone marrow, the spleen and the liver. There was no obvious infiltration of leukemic cells in lymph-nodes and thymus. In peripheral blood, there were a large number of leukemic cells and most of them were basophilic or polychromatophilic erythroblasts. At the advanced stage, the mice developed anemia and thrombocytopenia. The reaction of the leukemic cells for hemoglobin staining was positive or weakly positive. While the peroxydase reaction was negative, Thy-1 antigen and Fc-recepter were not presented. No virus-like particles was found in the cells under electron microscope. EL9611 leukemia model was sensitive to some antitumor drugs used in clinical therapy. CONCLUSION: The establishment of EL9611 leukemia model offered a useful tool for studying proliferation and differentiation of erythroid cells, as well as pathogenesis and experimental treatment of non-virus erythroid malignancy.