艾可康胶囊对白血病小鼠免疫功能的影响

目的:观察艾可康胶囊对Friend型白血病鼠的治疗作用.方法:BALB/c小鼠70只,分为正常对照组、模型对照组、齐多夫定对照组(0.1g·kg-1)、唐草片对照组(3.2 g·kg-1)、艾可康高、中、低剂量组(5,2.5,1.25 g·kg-1);除正常组外,每只小鼠ip 0.5 mL FLV病毒稀释液,正常组给予0.5 mL生理盐水.24 h后开始给药,正常对照组和模型对照组给予生理盐水,连续给药21 d.末次给药后取血,摘取脾脏.称取脾脏质量,计算脾指数,流式细胞仪测定CD4+,CD8+及CD4+,CD8+T淋巴细胞的幼稚细胞和记忆细胞.结果:与模型组相比较,艾可康高、中剂量组的脾指数、CD4+T淋巴细胞的记忆细胞均显著降低,CD4+,CD4 +/CD8+,CD4+T淋巴细胞的幼稚细胞百分率,CD8+T淋巴细胞的幼稚细胞百分率均显著升高(P＜0.05),艾可康高剂量组CD8+均显著升高,CD8+T淋巴细胞的记忆细胞百分率均显著降低(P＜0.05).结论:艾可康胶囊可提高Friend 型白血病鼠的免疫功能.

Effect of the Aikekang Capsule on Immunity in the Mice with Infection of Friend Leukemia Virus

Objective:To observe the therapeutic effect of the Aikekang capsule on the infection of Friend leukemia virus in mice. Method:Seventy BALB/c mice were randomly divided into seven groups:normal group,model group,Zidovudine group(0.1 g·kg^-1),Tangcaopian group(3.2 g·kg^-1), Aikekang groups (5,2.5, 1.25 g·kg^-1). Except for the normal group, the mice in other groups were inoculated intraperitoneally with Friend leukemia virus(FLV) to acquired immuno defiency syndrom (AIDS) model. Twenty-four hours after inoculation, the mice were given corresponding drugs according to the experimental design for 21 days. After treatment,the mice were executed and the effects of the Aikekang capsule on the spleen index, CD4⁺cells and CD8⁺cells, CD4⁺and CD8⁺T cells were determined. Result:High-dose and middle-dose Aikekang had reduced the spleen index and the percentage of CD4⁺memory T cell and rised the percentage of CD4⁺, the ratio of CD4⁺/CD8⁺, the percentage of CD4⁺immature T cell and the percentage of CD8⁺immature T cell; High-dose Aikekang increased the percentage of CD8⁺and reduced the percentage of CD8⁺memory T cell. Conclusion:The Aikekang capsule can enhance the immunity on the infection of Friend leukemia virus in mice.