活体人主动脉组织高迁移率族蛋白B1的表达及其与动脉粥样硬化发病机制的关系

目的 探讨活体人主动脉组织高迁移率族蛋白B1（HMGB1）的表达与动脉粥样硬化发病机制的关系。方法 36例冠状动脉旁路移植术（CABG）主动脉打孔时废弃的主动脉组织为研究组，12名活体肾移植供体者的动脉组织为对照组。两组患者的样本均应用免疫组化法检测动脉组织HMGB1表达；研究组患者术前测定血清总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）、脂蛋白(a)［Lp(a)］、载脂蛋白AⅠ（ApoAⅠ）、载脂蛋白B（ApoB）、高敏C-反应蛋白（hs-CRP）、总胆红素（TBIL）、直接胆红素（DBIL）、间接胆红素（IBIL）水平；采用冠状动脉造影、颈动脉超声检测冠状动脉及颈动脉的病变。结果 研究组主动脉组织HMGB1显著表达，而对照组动脉组织HMGB1无表达。研究组患者血清TC、TG、LDL-C、Lp(a)、ApoB、non-HDL-C、VLDL-C、hs-CRP测定值较参考值显著升高（P均＜0.01），而血清HDL-C、ApoAⅠ、TBIL、DBIL、IBIL测定值较参考值明显降低（P均＜0.01）；冠状动脉重度狭窄发生率为86.1%；颈动脉超声检测显示内膜中层厚度均＞1.2mm。结论 活体人主动脉组织HMGB1表达显著，表明动脉粥样硬化炎性反应明显；利用CABG主动脉打孔时废弃的主动脉组织为样本，为研究动脉粥样硬化发病机制及干预靶点提供了新的方法和途径。

Expression of human living aortic high mobility group protein box1 and its relationship with the pathogenesis of atherosclerosis

Objective To investigate the changes of high mobility group box1 (HMGB1) in the aorta of patients with coronary artery disease and its association with pathogenesis of atherosclerosis. Methods Aortic tissues from 36 patients undergoing coronary artery bypass graft (CABG) surgery were taken as the study group, while aortic tissues from 12 healthy subjects in the kidney donation program were taken as controls. Expression of HMGB1 was determined by immunohistochemistry. The preoperative plasma TC, TG, LDL-C, HDL-C, Lp (a), ApoA I, ApoB, non-HDL-C, VLDL-C, hs-CRP, TBIL, DBIL and IBIL of patients in the research group were examined. The coronary angiography and carotid artery ultrasound were performed in order to analyze the severity of atherosclerotic lesions. Results The HMGB1 protein was detectable in aortas from CABG patients. However, it was absent from the controlled tissues. The plasma TC, TG, LDL-C, Lp (a), ApoB, non-HDL-C, VLDL-C, and hs-CRP of patients in the research group were significantly elevated than the reference values (P<0.01), whereas plasma HDL-C, ApoA I, TBIL, DBIL and IBIL were significantly lower than the reference values (P<0.01). The incidence of severe coronary artery stenosis was 86.1% and intima-media thickness was more than 1.2mm. Conclusion Over-expression of HMGB1 in atherosclerosis aortic tissues indicates that inflammatory reaction is obvious in atherosclerosis mottling. Taking aortic tissues from CABG as samples may be a new method in the study of atherosclerosis.