三七三醇皂苷对糖尿病大鼠视网膜神经节细胞的保护作用

【摘要】目的探讨三七三醇皂苷（PTS）对糖尿病大鼠视网膜神经节细胞（RGC）的保护作用及机制。方法SD大鼠随机分为对照组、糖尿病组及治疗组，糖尿病组及治疗组腹腔注射链脲佐菌素诱导糖尿病动物模型，治疗组给予PTS50mg·kg-1·d-1灌胃，1个月后免疫荧光组化双标检测Nogo受体与RGC特异性标志物Brn3a的共存情况，Western blot检测视网膜Nogo受体表达，试剂盒检测视网膜丙二醛（MDA）含量，HE染色观察RGC数量。结果Brn3a与Nogo受体于视网膜内大量共存。与对照组相比，糖尿病组视网膜Nogo受体表达上调，MDA含量增加，RGC数量减少（均P＜0.001）；与糖尿病组相比，治疗组视网膜Nogo受体表达减少，MDA含量降低，RGC数量增加（P＜0.001）。结论PTS可能通过抑制糖尿病大鼠视网膜RGC内Nogo受体的表达，抑制视网膜氧化应激，从而保护糖尿病视网膜RGC。

Protective Effects of Panax Notoginseng on Retinal Ganglion Cells in Diabetic Rats

Abstract]ObjectiveTo explore protective effects and potential underlying mechanisms of panax notoginseng(PTS) on retinal ganglion cell (RGC) in diabetic rats.MethodsSD rats were randomly divided into control group, diabetic group and treatment group. The diabetic model rats were induced by intraperitoneal injection of streptozotocin. Rats were given PTS50mg·kg-1·d-1in treatment group. One month later, the coexistence of nogo receptor and Brn3a (special marker ofRGC) was observed by immunofluorescence staining double-labeled method. The expression of Nogo receptor was detectedby Western blot assay. The level of malondialdehyde (MDA) in retina was measured with detection kit. HE-staining was in?troduced to reveal the number of retinal RGC.ResultsA large number of Brn3a and Nogo receptors were co-existed in the retina. The Nogo receptor was exclusively expressed in RGC, which was up regulated in diabetic group compared with that ofcontrol group. The level of retinal MDA was increased and the number of RGC decreased in diabetic group than that of control group (P＜0.001). Compared with diabetic group, there were decreased retina Nogo receptor, decreased level of MDA and increased number of RGC in treatment group (P＜0.001).Conclusion PTS attenuates diabetes-induced loss of RGC,which may ascribe for down-regulation of retina Nogo receptor and decreased oxidative stress