二甲亚砜促进5-氨基酮戊酸光动力疗法对A431细胞杀伤和小鼠皮肤鳞癌的抑制效应

目的:研究DMSO参与5-氨基酮戊酸介导的光动力疗法（ALA-based photodynamic therapy，ALA-PDT）对A431细胞杀伤和小鼠皮肤鳞癌的抑制效应，探讨治疗皮肤肿瘤的优化作用。方法:DMSO干预后，采用流式细胞仪检测A431细胞内PpⅨ水平和PDT后细胞凋亡率。激光共聚焦显微镜检测小鼠鳞癌瘤体组织的荧光强度，比较PDT后各组鳞癌小鼠的抑瘤率。结果:实验组A431细胞PpⅨ荧光强度率高于ALA组和空白对照组（P<0.01），与DMSO含量呈浓度依赖性。PDT后实验组细胞凋亡率高于ALA组和空白对照组（P<0.01）。实验组瘤体组织PpⅨ荧光强度值高于10%ALA对照组和10%DMSO对照组（P<0.01），与DMSO含量呈浓度依赖性。实验组鳞癌小鼠 PDT治疗后，抑瘤率高于对照组（P<0.05），并有量效依从关系。结论:DMSO可促进ALA-PDT对A431细胞杀伤和小鼠皮肤鳞癌的抑制效应。

Enhanced 5-Aminolevulinic acid-based photodynamic effects by Dimethyl sulfoxide on A431 cytotoxicity in vitro and inhibition of skin squamous carcinoma in mice

Objective:To study the effect of enhanced 5-Aminolevulinic acid-based photodynamic therapy（ALA-PDT） by DMSO on A431 cytotoxicity in vitro and inhibition of mice skin squamous cell carcinoma（MSCC）in vivo.Methods:A431 cells were co-cultured with DMSO.Apoptosis after ALA-PDT and protoporphyrin Ⅸ（PpⅨ） concentration of A431 cells co-cultured with DMSO were detected by flow cytometry.The fluorescence intensity in MSCC frozen sections was detected by laser scanning confocal microscopy.After ALA-PDT the MSCC suppressing rates were compared.Results:The PpⅨ fluorescence intensity of A431 cells in the experimental/therapeutic group showed DMSO dose dependant manner，was higher than that in the ALA alone or control group(P<0.01).After ALA-PDT，the A431 cell apoptotic rates in the experimental group were higher than that in the ALA alone or control group(P<0.01).The PpⅨ fluorescence intensity in the experimental group showed DMSO dose dependant manner was higher than that in the ALA alone or control group(P<0.01).After ALA-PDT the tumor suppressing rates showed DMSO dose dependant manner in therapeutic group(P<0.05).Conclusion:DMSO can promote ALA-PDT effects on A431 cytotoxicity and MSCC inhibition.