Beclin-1与Bcl-2在血管性痴呆大鼠海马CA1区的表达及意义

【摘要】目的观察自噬相关蛋白Beclin-1与凋亡相关蛋白Bcl-2在血管性痴呆大鼠海马CA1区的表达及变化情况。方法60只实验大鼠随机分为假手术组和血管性痴呆模型组（模型组），每组又随机分为模型制备成功后1、2、4、8和12周5个亚组（各6只）。采用四血管阻断法制备血管性痴呆模型，Morris水迷宫法测试学习记忆能力，免疫组化法检测自噬相关蛋白Beclin-1及凋亡相关蛋白Bcl-2的表达。结果模型组大鼠海马CA1区1周时可见Be clin-1、Bcl-2蛋白阳性表达，4 周达到高峰，8周开始下降，至12 周仍较高。与假手术组比较，模型组各时间点的Be clin-1、Bcl-2蛋白表达均明显增高，差异均有统计学意义（P < 0.05或P < 0.01）。模型组大鼠海马CA1区的Beclin-1阳性表达数与Bcl-2阳性表达数呈正相关（r=0.809，P < 0.05）。结论血管性痴呆大鼠海马CA1区自噬和凋亡同时发生，且表达趋势一致。

Clinical Significance of Beclin-1and Bcl-2Expressions in CA1Area of Hippocampus in Vascular Dementia Rats

Abstract]ObjectiveToobservethe expressions of autophagy-related proteins Beclin-1and apoptosis-related pro?teins Bcl-2in CA1area of hippocampus in vascular dementia model rats.MethodsThe experimental rats were randomly divided into sham operation group and vascular dementia model group. The model group was divided into five subgroups:1,2, 4, 8and12- w group (n=6for each group). The vascular dementia rat model was established by blocking four vessels.Learning and memory abilities were detected by Morris water maze. The expressions of Beclin-1and Bcl-2were detected by immunohistochemistry assay. Results The positive expressions of Beclin-1and Bcl-2were detected at1-w in CA1area of hippocampus in model rats, which reached the peak at4-w and began to decline in8-w, maintained the higher levels at12-w. There were significantly higher expressions of Beclin-1and Bcl-2at all-time points in model group than those of sham operational group (P<0.05orP<0.01). The positive expression of Beclin-1was positively correlated with Bcl-2at alltime points in CA1area of hippocampus in model group (r=0.809, P<0.05). Conclusion Results showed that autophagy and apoptosis occurred simultaneously and expressed the same trend in CA1area of hippocampus of vascular dementia model rats.