血管内皮细胞对牙周组织细胞增殖的影响

目的：通过血管内皮细胞与牙周组织细胞复合培养模拟牙周组织再生环境，观察血管内皮细胞对牙周组织细胞增殖的作用规律，探讨血管内皮细胞对牙周组织修复再生的影响。方法：应用原代培养的血管内皮细胞，在Transwell嵌套中实现与人牙周膜成纤维细胞、牙龈成纤维细胞间的复合培养，分别于复合培养第2、4、6、8和10天，以细胞计数手段检测血管内皮细胞共存条件下2种牙周组织细胞的增殖情况，并与单独培养的2种牙周组织细胞作为对照，采用SPSS13.0软件包对数据进行统计学分析。结果：在血管内皮细胞存在条件下，2种牙周组织细胞的增殖速率均显著高于单独培养条件。血管内皮细胞存在时，牙周膜成纤维细胞增殖速率逐渐超越牙龈成纤维细胞(第6天起)，并在第8天细胞数量上超过牙龈成纤维细胞，差异具有显著性(P<0.01)。结论：血管内皮细胞的存在，对牙周组织细胞的增殖具有促进作用，对牙周膜成纤维细胞增殖的促进作用显著高于牙龈成纤维细胞。

The effect of vascular endothelial cells on the proliferation of periodontal ligament cells and gingival fibroblasts

PURPOSE: To investigate the effect of vascular endothelial cells on the proliferation of periodontal ligament cells and gingival fibroblasts through a model mimicking the periodontal wound healing and regeneration. METHODS: Human vascular endothelial cells, periodontal ligament cells and gingival fibroblasts were co-cultured using a Transwell system. The number of each type of cells at 2, 4, 6, 8 and 10 days after co-culture were counted using a cell counter machine, while periodontal ligament cells and gingival fibroblasts single culture were used as control. The results were statistically processed with SPSS 13.0 software package. RESULTS: When vascular endothelial cell was present, the proliferation of periodontal ligament cells and gingival fibroblasts were faster than each single culture, the difference between them was significant (P<0.01). The proliferation of periodontal ligament cells was faster than gingival fibroblast as they were co-cultured with vascular endothelial cells(which happened at day 6), although the results were contrary when they were single cultured, the difference between them was significant (P<0.01). CONCLUSIONS: The presence of vascular endothelial cells can promote the proliferation of periodontal cells, and the effect is more prominent on human periodontal ligament cells than on gingival fibroblasts.