| 5种牙本质黏结剂对人牙周膜细胞毒性的比较 |
| |
| 引用本文： | 李俊亮,王文梅,葛久禹,季骏,王天丛.5种牙本质黏结剂对人牙周膜细胞毒性的比较[J].上海口腔医学,2013,22(6):628-633. |
| |
| 作者姓名： | 李俊亮 王文梅 葛久禹 季骏 王天丛 |
| |
| 作者单位： | (1. 南京大学医学院附属口腔医院 牙体牙髓病科，2.口腔正畸科，南京大学口腔医学院 中心实验室，江苏 南京 210008) |
| |
| 基金项目： | 江苏省医学领军人才与创新团队项目(LJ201110)；江苏省自然科学基金(SBK201340904) |
| |
| 摘 要： | 目的：比较5种牙本质黏结剂对人牙周膜细胞的毒性作用。方法：原代培养人牙周膜细胞，采用四甲基偶氮唑盐比色法(MTT)和浸提液法，观察5种牙本质黏结剂(Super Bond C&B、Clearfil SE Bond、G-Bond、Single Bond2、Adper Easy One)不同体积分数浸提液(100%、50%、25%)作用不同时间(24 h、48 h、72 h)对人牙周膜细胞的毒性作用。采用SPSS19.0软件包对数据进行统计学分析。结果：5种牙本质黏结剂对人牙周膜细胞的细胞毒性Super BondP<0.05)，48 h与72 h无显著差异(P>0.05)。结论：5种牙本质黏结剂细胞毒性程度不同，Super Bond C&B、G-Bond和Clearfil SE Bond的细胞毒性均较轻微，Single Bond2的细胞毒性较强。
|
| 关 键 词： | 牙本质黏结剂 人牙周膜细胞 细胞毒性 |
| 收稿时间： | 2013-08-22 |
| 修稿时间： | 2013-09-23 |
A comparative study of the cytotoxicity of five dental bonding agents to human periodontal ligament cells |
| |
| LI Jun-liang,WANG Wen-mei,GE Jiu-yu,JI Jun,WANG Tian-cong.A comparative study of the cytotoxicity of five dental bonding agents to human periodontal ligament cells[J].Shanghai Journal of Stomatology,2013,22(6):628-633. |
| |
| Authors: | LI Jun-liang WANG Wen-mei GE Jiu-yu JI Jun WANG Tian-cong |
| |
| Institution: | 1.Department of Endodontics, 2. Department of Orthodontics, Nanjing Stomatological Hospital, Central Laboratory of Stomatological School of Nanjing University. Nanjing 210008, Jiangsu Province, China |
| |
| Abstract: | PURPOSE: To compare the cytotoxicity of 5 different dental bonding agents to human periodontal ligament cells(HPDLCs). METHODS: Primary cultured HPDLCs were exposed to different concentrations(100%，50% and 25%)of Super Bond, Clearfil SE Bond, G-Bond, Single Bond2, and Adper Easy One for different times (24 h, 48 h and 72 h). Cytotoxicity of 5 different agents to HPDLCs were observed by MTT method. The data was analyzed using SPSS 19.0 software package. RESULTS: Cytotoxicity of 5 dentin bonding agents to HPDLCs were Super BondP<0.05)but no significant difference was found between 48h and 72h(P>0.05). CONCLUSIONS: Cytotoxicity of 5 dentin bonding agents are different. Cytotoxicity of Super-bond C&B，G-Bond and Clearfil SE Bond are weak, while cytotoxicity of Single Bond2 is the strongest among the 5 agents. |
| |
| Keywords: | Dental bonding agents HPDLCs Cytotoxicity |
|
| 点击此处可从《上海口腔医学》浏览原始摘要信息 |
| 点击此处可从《上海口腔医学》下载免费的PDF全文 |
