中波紫外线诱导小鼠皮肤光损伤中miRNA141表达的研究

【摘要】 目的 探讨小室移植法重建小鼠毛囊，观察细胞成分对毛囊再生的影响。 方法 取0 ～ 2 d的C57BL/6乳鼠背部皮肤，胰酶消化后分离表真皮，再分离出毛囊上皮细胞。实验分表皮细胞混合毛囊胚芽组、真皮细胞组、表皮细胞混合毛囊胚芽 + 真皮细胞组、毛囊上皮细胞 + 真皮细胞组。用小室移植法接种于裸鼠背部，并于移植后1、2、4、8周观察变化，HE染色观察毛囊组织学形态。 结果 小鼠毛囊细胞移植1周时，背部小室开始脱落，伤口结痂；2周时除表皮细胞组外，另3组均长出了短小的毛发，镜下可见毛囊样结构；4周及8周时除表皮细胞组外，均长出了正常的毛发，表皮细胞与真皮细胞混合组及毛囊上皮细胞与真皮细胞混合组毛发生长情况良好，优于单独的真皮细胞组。 结论 毛囊细胞小室移植后可形成新的毛囊，上皮细胞及真皮细胞在毛囊重建中具有重要的作用。 【关键词】 毛囊； 毛囊重建； 鼠科

Reconstruction of hair follicles in mice: a pilot study

ZHANG Xiao-yu, WANG Da-guang, ZHU Feng, YUE Xue-zhuang, LUO Dan. Department of Dermatology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China Corresponding author: WANG Da-guang, Email: wangirwin@126.com 【Abstract】 Objective To reconstitute hair follicles in mice using graft chambers, and to study the effect of different cell types on hair follicle regeneration. Methods Full-thickness skin was obtained from the back of C57BL/6 neonatal mice. Then, epidermal cell suspensions were prepared by shredding epidermis after trypsinization, hair follicles and dermal cells were collected by filtration, low-speed centrifugation and density gradient centrifugation, and hair follicle epithelial cells were obtained via trypsinization of hair follicles followed by filtration. Nude mice were classified into four groups to be transplanted with epidermal cells + follicular buds, dermal cells alone, epidermal cells + follicular buds + dermal cells, follicular epithelial cells + dermal cells, respectively. The cells were implanted into the dorsal skin of nude mice using fold chambers. After the grafting, the growth of skin and hairs was observed at the grafted sites on week 1, 2, 4 and 8, and skin specimens were obtained on week 2, 4, and 8 for histological study of hair follicles using hematoxylin-eosin staining. Results After grafting, the chambers on the back of nude mice began to shed with crust formation on week 1; stunted hairs came out and follicle-like structures were seen under the microscope on week 2 at the grafted sites, normal hairs were observed on week 4 and 8 in all the mice except for those transplanted with epidermal cells + follicular buds, and the growth of hairs in mice grafted with epidermal cells + follicular buds + dermal cells and mice grafted with follicular epithelial cells + dermal cells was superior to that in mice grafted with dermal cells alone. Conclusions Hair follicles can regenerate after hair follicle cell transplantation into dorsal chambers in nude mice. Both epidermal cells and dermal cells play indispensable roles in hair follicle reconstitution. 【Key words】 Hair follicle; Hair follicle reconstitution; Muridae