Effects of Bioreactor‐Oxygenation During Extracorporeal Granulocytes Treatment in Septic Patients |
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| Authors: | Martin Sauer Georg Richter Jens Altrichter Thomas Wild Fanny Doß Thomas Mencke Johannes Ehler Sandra Doß Stephanie Koch Anton Schubert Gabriele Nöldge‐Schomburg Steffen R Mitzner |
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| Institution: | 1. Department of Anesthesiology and Intensive Care Medicine, University Hospital of Rostock, Rostock, Germany;2. Fraunhofer Institute for Cell Therapy and Immunology, Rostock, Germany;3. Artcline GmbH, Rostock, Germany;4. Department of Surgery, University Hospital of Rostock, Rostock, Germany;5. Department of Medicine (Division of Nephrology), University Hospital of Rostock, Rostock, Germany |
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| Abstract: | A granulocyte bioreactor for the extracorporeal treatment was developed to enhance the immune cell function in patients with severe sepsis. The influence of oxygenation on the used cells was tested in a prospective clinical study. Ten patients with severe sepsis were treated twice with the granulocyte bioreactor. The used cells were screened for functionality; values of blood gases, glucose and lactate were obtained from the recirculating bioreactor circuit. Five patients were treated with an oxygenator setup (Oxy group), five without oxygenator (Non‐Oxy group). The overall in‐hospital mortality was 50%. Significantly lower values of oxygen saturation, partial oxygen pressure, lactate, oxyburst and phagocytosis were seen in the Non‐Oxy group compared with the Oxy group in the bioreactor circuit. Further studies with this approach are encouraged and should focus on the influence of oxygenation on production of reactive oxygen species and cytokines of used cells. |
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| Keywords: | Extracorporeal circulation Neutrophils Oxygenator Plasmapheresis Severe sepsis |
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