pEgr-angiostatin基因辐射诱导表达特性及其抗肿瘤作用

目的 :检测 p Egr- angiostatin重组质粒在 B1 6细胞中的辐射诱导表达和观察 p Egr-angiostatin基因 -放射治疗的抗肿瘤作用。方法 :用 RT- PCR方法从小鼠肝脏中扩增出 angiostatinc DNA,经测序证实后 ,构建 p Egr- angiostatin重组质粒 ,脂质体介导的转染法转染小鼠 B1 6细胞 ,检测 B1 6细胞内 angiostatin m RNA的辐射诱导表达 ,体内观察 p Egr- angiostatin基因 -放射治疗的抑瘤作用。结果 :测序表明扩增的 angiostatin c DNA序列与报道基本一致 ,Egr- 1启动子和 angiostatinc DNA正确插入表达载体 pc DNA3.1 ,转染 B1 6细胞内 angiostatin m RNA的表达具有辐射诱导特性 ,p Egr- angiostatin基因 -放射治疗荷瘤小鼠具有明显的抑瘤作用。结论 :成功地克隆小鼠angiostatin基因 ,并证实 p Egr- angiostatin的辐射诱导表达规律及其基因 -放射治疗的抗肿瘤作用

Expression Property and Antitumor Effect of pEgr-angiostatin Induced by Ionizing Radiation

Objective:To study on the expression and antitumor effect of pEgr angiostatin induced with ionizing radiation. Methods:Mouse angiostatin cDNA was amplified with RT PCR. The pEgr angiostatin recombinant plasmid was constructed and was then transfected into B16 cells with liposome. The cells were irradiated and the expression of angiostatin mRNA in B16 cells was detected. Finally, the antitumor effect of pEgr angiostatin was studied. Results:The sequence of mouse angiostatin cDNA was identical to reported. Egr 1 promoter and angiostatin cDNA was inserted correctly into expression vector. The expression property of pEgr angiostatin was examined by ionizing radiation. The gene therapy with pEgr angiostatin showed remarkably antitumor effect.Conclusion:Mouse angiostatin cDNA had been cloned successfully in the study.The expression property and the antitumor effect of pEgr angiostatin are induced by ionizing radiation. This result provides the basis for multi gene radiotherapy of tumor.