不同剂量重组人生长激素对2型糖尿病大鼠创面愈合的影响

目的探讨重组人生长激素(rhGH)对T2DM大鼠创面愈合的影响。方法建立T2DM大鼠创面模型,32只大鼠随机分为对照组(T2DM)、rhGH 0.2 IU/kg组(rhGH 0.2)、rhGH0.4 IU/kg组(rhGH 0.4)、rhGH 0.6 IU/kg组(rhGH 0.6),每组8只,各组分别予0.9%生理盐水和0.2、0.4、0.6 IU/kg rhGH干预14 d。观察各组第0、3、7、14天时平均残余创面面积及创面完全愈合时间,检测干预前后血糖水平,HE染色观察创面组织病理变化。结果 14 d时,rhGH 0.2、rhGH 0.4、rhGH 0.6组残余创面面积小于T2DM组(P<0.05),rhGH 0.2、rhGH 0.4、rhGH 0.6组残余创面面积依次减小(P<0.05),rhGH 0.4、rhGH 0.6组平均创面完全愈合时间短于T2DM、rhGH 0.2组(P<0.05)。各组血糖趋势随时间递增,各组间相同时间点血糖水平比较差异无统计学意义(P>0.05)。HE染色结果显示,T2DM、rhGH 0.2组创面组织内炎性细胞浸润较多;rhGH 0.4、rhGH 0.6组上皮化水平及胶原形成较好,创面愈合程度高。结论 0.6 IU/kg剂量rhGH能缩短T2DM大鼠创面慢性炎症浸润时间,加速创面愈合速度,予小剂量rhGH治疗DM创面时,不会引起血糖明显升高。

Effects of different doses of recombinant human growth hormone on wound healing in type 2 diabetic rats

Objective To investigate the effect of recombinant human growth hormone(rhGH) on wound healing in T2DM rats.Methods Wounded model of T2DM rats was established.32 rats were randomly divided into control group(T2DM), rhGH 0.2 IU/kg group(rhGH 0.2), rhGH 0.4 IU/kg group(rhGH 0.4) and rhGH 0.6 IU/kg group(rhGH 0.6).Eight rats in each group.They were treated with 0.9% normal saline,0.2,0.4 and 0.6 IU/kg rhGH for 14 days,respectively.The average residual wound area and the time to complete wound healing were observed at 0,3,7 and 14 d in each group.blood glucose level was detected before and after intervention,and the histopathological changes of wound were observed by HE staining.Results At 14 d,the residual wound area was smaller in rhGH 0.2,rhGH 0.4 and rhGH 0.6 groups than in T2DM group(P<0.05).Residual wound area decreased successively in rhGH0.2,rhGH 0.4 and rhGH 0.6 groups(P<0.05).Mean wound healing time was shorter in rhGH 0.4 and rhGH 0.6 group than in T2DM and rhGH 0.2 group(P<0.05).The blood glucose trend increased with time in each group,and there was no significant difference in the blood glucose trend among all groups(P>0.05).HE staining results showed that T2DM and rhGH 0.2 group had more inflammatory cell infiltration in the wound tissue.The rhGH 0.4 and rhGH 0.6 groups had better epithelialization and collagen formation,and the wound healing degree was high.Conclusion rhGH at 0.6 IU/kg dose could shorten the time of chronic inflammatory infiltration on the wound surface in T2DM rats and accelerate the speed of wound healing.Small dose of rhGH did not cause significant increase of blood glucose on the wound surface of DM rats.