维生素D通过维生素D受体-过氧化物酶体增殖物激活受体γ信号通路对高糖诱导的巨噬细胞血管内皮生长因子C表达的影响 |
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引用本文: | 罗丽娅,阳琰,蔡玉兰,王雪梅,魏在荣,牟芝群,岳瑜,朱玉玉,胡皓铭,姚扬,吴旻,邓嘉杰.维生素D通过维生素D受体-过氧化物酶体增殖物激活受体γ信号通路对高糖诱导的巨噬细胞血管内皮生长因子C表达的影响[J].中华糖尿病杂志,2021(3):215-220. |
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作者姓名: | 罗丽娅 阳琰 蔡玉兰 王雪梅 魏在荣 牟芝群 岳瑜 朱玉玉 胡皓铭 姚扬 吴旻 邓嘉杰 |
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作者单位: | 遵义医科大学附属医院内分泌科;遵义医科大学附属医院烧伤整形科 |
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基金项目: | 国家自然科学基金(82060159);贵州省自然科学基金资助项目(黔科合基础[2020]1Y314);遵义医学院新苗培养及创新探索专项后补助课题(黔科合平台人才[2017]5733-043);遵义市联合基金(遵市科合社字[2018]63)。 |
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摘 要: | 目的探讨维生素D(VitD)对高糖诱导的巨噬细胞(MO)内维生素D受体(VDR)、PPARγ、血管内皮生长因子C(VEGFC)表达的影响。方法通过前期浓度及时间依赖实验,以浓度25 mmol/L葡萄糖依赖性培养小鼠MO系(U937)24 h。实验分为对照组(Con)、VitD组、PPARγ抑制剂(T0070907)组、VitD+T0070907组。ELISA法检测TNF-a、IL-6、VEGFC水平,实时荧光定量PCR、Western blot法分别检测VDR、PPARγ、VEGFC基因及蛋白表达水平。结果VitD呈浓度、时间依赖性表达增加MO内TNF-α、IL-6、VEGFC水平,在VitD浓度为0.1 mmol/L作用24 h产生最大效应。与Con组比较,VitD组VDR、PPARγ、VEGFC基因及蛋白表达水平升高(P<0.05)。与VitD组比较,T0070907组、VitD+T0070907组PPARγ、VEGFC基因及蛋白表达水平减少(P<0.05)。结论VitD可能通过VDR-PPARγ信号通路,促使MO内VDR、PPARγ、VEGFC表达增加,减轻高糖环境下MO炎症反应。
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关 键 词: | VDR-PPARγ信号通路 维生素D受体 过氧化物酶体增殖物激活受体Γ 血管内皮生长因子C 巨噬细胞 |
Effects of vitamin D on the expression of VDR,PPARγand VEGFC in macrophages induced by high glucose through VDR-PPARγsignaling pathway |
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Institution: | (Department of Endocrinology,Affiliated Hospital Zunyi Medical University,Zunyi 563000,China) |
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Abstract: | Objective To investigate the effect of vitamin D(VitD)on the expression of vitamin D receptor(VDR),peroxisome proliferator activated receptorγ(PPARγ)and vascular endothelial growth factor C(VEGFC)in macrophages(MO)induced by high glucose.Methods Based on the previous concentration and time-dependent experiments,25 mmol/L glucose was selected to culture mouse Mo line(U937)for 24 h.The experiment was divided into control group(Con),Vit D group,T0070907 group and Vit D+T0070907 group.The levels of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and VEGFC were detected by ELISA.The expression levels of VDR,PPARγ,VEGFC gene and protein were detected by RT-PCR and Western blot.Results VitD increased the levels of TNF-a,IL-6 and VEGFC in Mo in a concentration and time-dependent manner.The maximum effect was observed at the concentration of 0.1 mmol/L for 24 h.Compared with con group,VDR,PPARγ,VEGFC gene and protein expression were increased in Vit D group(P<0.05).Compared with Vit D group,the expression of PPARγ,VEGFC protein and gene in T0070907 group and Vit D+T0070907 group were decreased(P<0.05).Conclusion VitD may increase the expression of VDR,PPARγand VEGFC in Mo through VDR-PPARγsignaling pathway,and reduce the inflammatory response of Mo in high glucose environment. |
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Keywords: | VDR-PPARγsignaling pathway Vitamin D receptor Peroxisome proliferator activated receptorγ Vascular endothelial growth factor C Macrophages |
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