| RNAi抑制CYP3A4基因表达的实验研究 |
| |
| 引用本文： | 杨贵忠,余华荣,袁野,周岐新,刘颖菊.RNAi抑制CYP3A4基因表达的实验研究[J].南方医科大学学报,2008,28(12):2227-2229. |
| |
| 作者姓名： | 杨贵忠 余华荣 袁野 周岐新 刘颖菊 |
| |
| 作者单位： | 1. 重庆医科大学基础医学院药理教研室,重庆,400016
2. 重庆医科大学生理教研室,重庆,400016 |
| |
| 摘 要： | 目的 探讨质粒表达型短发夹RNA(shRNA)对CHL-3A4转基因细胞的细胞色素P450 3A4(CYP3A4)基因表达的影响.方法 构建3条shRNA真核表达载体(CYP3A4 Ⅰ、CYP3A4 Ⅱ、CYP3A4 Ⅲ),脂质体转染CHL-3A4转基因细胞.转染48h后,RT-PCR和Western blotting分别观察3条shRNA对CYP3A4mRNA和蛋白表达的影响;噻唑蓝观察shRNA抑制环磷酰胺对CHL-3A4转基因细胞毒性作用.结果 CYP3A4 Ⅲ表达载体的shRNA分别能抑制CHL-3A4细胞的CYP3A4mRNA表达(75%)及蛋白表达(80%),抑制环磷酰胺对CHL-3A4细胞(75%)细胞毒作用.结论 RNA干扰能显著抑制CYP3A4基因的表达,RNA干扰技术为肝细胞色素P450的研究提供了新的实验室方法 .
|
| 关 键 词： | RNA于扰 细胞色素P450 基因表达 环磷酰胺 |
RNA interference-mediated silencing of cytochrome P450 3A4 gene in CHL-3A4 cells |
| |
| YANG Gui-zhong,YU Hua-rong,YUAN Ye,ZHOU Qi-xin,LIU Ying-ju.RNA interference-mediated silencing of cytochrome P450 3A4 gene in CHL-3A4 cells[J].Journal of Southern Medical University,2008,28(12):2227-2229. |
| |
| Authors: | YANG Gui-zhong YU Hua-rong YUAN Ye ZHOU Qi-xin LIU Ying-ju |
| |
| Institution: | YANG Gui-zhong1,YU Hua-rong2,YUAN Ye1,ZHOU Qi-xin1,LIU Ying-ju1 1Department of Pharmacology,2Department of Physiology,Chongqing Medical University,Chongqing 400016,China |
| |
| Abstract: | Objective To investigate the effect of small hairpin interfering RNA (shRNA) in suppressing cytochrome P450 3A4 (CYP3A4) gene expression in CHL-3A4 cells. Methods Three shRNA expression vectors targeting CYP3A4 gene (CYP3A4 I, C YP3A4 II, and CYP3A4 III, respectively) were designed, synthesized and transfected into CHL-3A4 cells via liposomes. The inhibitory effect of shRNA on CYP 3A4 gene expression was detected by Western blotting and RT-PCR, and the effect of shRNA transfection in suppressing cyclophosph... |
| |
| Keywords: | RNA interference cytochrome P450 gene expression cyclophosphamide |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《南方医科大学学报》浏览原始摘要信息 |
| 点击此处可从《南方医科大学学报》下载免费的PDF全文 |
|
