儿宝颗粒对厌食大鼠下丘脑外侧区神经元整合摄食相关信息的影响

目的 证实儿宝颗粒影响下丘脑外侧区神经元对外周传入信号的敏感性,阐明儿宝颗粒促进摄食行为的中枢机制.方法 用特制饲料喂养大鼠1周制备厌食模型,用儿宝颗粒灌胃治疗3周,然后用电生理技术细胞外记录法,记录大鼠下丘脑外侧区神经元的自发放电,观察其对电刺激胃迷走神经和静脉注射葡萄糖注射液的反应,比较正常组、模型组和治疗组3组间的异同.结果 造模7天时模型组和治疗组大鼠进食量低于正常组（P＜0.01）,造模21、28天时治疗组大鼠进食量与正常组比较差异无显著性.与正常组比较,模型组那些接受胃迷走神经传入的下丘脑外侧区神经元中,血糖敏感神经元所占比例显著降低（P＜0.01）;而治疗组该指标与正常组比较差异无显著性.结论 儿宝颗粒能够调节摄食中枢神经元对外周传入信号的敏感性,从而影响摄食中枢神经元对多种传入信息的整合.

Effect of Er'bao Granule on Integration of Ingestion Behavior-related Information by Neurons in Lateral Hypothalamic Area of Anorexia Rats

ObjectiveTo confirm the effect of Er'bao granule (EBG) on the sensitivity to peripheral afferent signal of neurons in lateral hypothalamic area (LHA) to illustrate the central mechanism of EBG in promoting ingestion behavior. Methods The anorexia rat model was established by feeding special prepared forage for one week, and all the model rats were administrated with EBG by gavage for 3 weeks. The spontaneous discharge of LHA neurons was recorded using electro-physiological extracellular recording method, and its response to electrical stimulus on gastric vagus nerve and intravenous injection of glucose were observed and compared among the normal, model and treated groups. Results As compared with the normal group, among the LHA neurons responding to afferent gastric vagal impulse, the proportion of glycemia-sensitive neurons in the model group was significantly decreased (P<0.01), but insignificant difference was shown in comparison between the treated group and the normal group. Conclusion EBG play a role in regulating the sensitivity of LHA neurons to peripheral afferent signal and thus to influence the multi-afferent information integration of ingestion central neurons.