| HPLC法测定定痛宁冲剂中阿魏酸的含量 |
| |
| 引用本文： | 顾玉英,汪怡,尤奋强,王宏源.HPLC法测定定痛宁冲剂中阿魏酸的含量[J].安徽医药,2013,17(11):1873-1874. |
| |
| 作者姓名： | 顾玉英 汪怡 尤奋强 王宏源 |
| |
| 作者单位： | 南京中医药大学附属昆山中医医院,江苏,昆山,215300;南京中医药大学附属昆山中医医院,江苏,昆山,215300;南京中医药大学附属昆山中医医院,江苏,昆山,215300;南京中医药大学附属昆山中医医院,江苏,昆山,215300 |
| |
| 摘 要： | 目的建立HPLC法测定定痛宁冲剂中阿魏酸含量的方法。方法采用HPLC法测定阿魏酸含量，以Diamonsil C18柱（5μm，4．6mm×250mm）为色谱柱；甲醇：1％醋酸溶液（30：70）为流动相；检测波长：321nm。结果阿魏酸在进样量0．212～1．060μg范围内呈良好的线性关系，回归方程为Y=4．6855×10^6X-2．1090×10^4（r=0．9999），平均加样回收率98．96％，RSD=1．92％。结论该方法操作简单，方法可靠，结果准确，灵敏度高，重现性好，可作为该产品质量控制的方法。
|
| 关 键 词： | 阿魏酸 定痛宁冲剂 HPLC法 |
Deternination of ferulaic acid in dingtongning granules by HPLC |
| |
| Institution: | GU Yu-ying,WANG Yi,YOU Fen-qiang, et al ( Kunshan Hospital of TCM,Kunshan ,Jiangsu 215300, China) |
| |
| Abstract: | Objective To establish an HPLC method for determination of ferulaic acid in Dingtongning granules. Methods HPLC meth- od was applied to determine the content of ferulaic acid, with Diamonsil Cls column （5 μm,4.6 mm × 250 mm） as chromatographic column;The mobile phase consisted of methanol： glacial acetic acid-water solution （30：70）. The detection wavelength was 321 nm. Results Ferulaic acid showed a good linear relationship at a range of 0.212 - 1. 060 μg. The regression equation was Y = 4. 685 5 × 10^6X - 2. 109 0 × 10^4 （r = 0.999 9）. The average recoveries were 98.96%. RSD was 1.92%. Conclusions This method has good repeat- ability, flexibility and high sensitibity. It can be used for quality control of Dingtongning granules. |
| |
| Keywords: | ferulaic acid dingtongning granules HPLC |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
