吡咯列酮对HDL功能的调节及其抗动脉粥样硬化的机制

目的分析在动脉粥样硬化状态下过氧化物酶体增殖物激活受体激动剂（PPARl）吡咯列酮对肝细胞、腹腔巨噬细胞ATP结合盒转运子AI（ATP binding cassette transporter 1，ABCA1）、B族I型清道夫受体（Scavenger receptorclass BtypeI，sR—BI）及其3H]胆固醇转出率、血浆炎性因子水平的影响，探讨吡咯列酮在胆固醇逆向转运过程中对HDL功能的调节及其抗动脉粥样硬化的机制。方法将30只雄性日本大耳兔随机分为3组。（1）对照组（n=10）：正常饮食喂养；（2）动脉粥样硬化（AS）组（n=10）：单纯喂高胆固醇饲料；（3）吡咯列酮组（n=10）：高胆固醇饮食的基础上予以喂服吡咯列酮。20周后测定兔血脂、外周肝细胞以及腹腔巨噬细胞表面ABCAl和SR-BI的表达、胆固醇转出率以及血清炎性因子浓度。结果与正常对照组相比，AS组肝细胞和腹腔巨噬细胞的ABCA1、SR—B1及3H]胆固醇醇转出率表达均明显降低，其差异均有统计学意义；与AS组相比，吡咯列酮组肝细胞和腹腔巨噬细胞的ABCA1、SR．B1、3H]胆固醇醇转出率表达均显著升高，其差异均有统计学意义。结论吡格列酮可通过增加腹腔巨噬细胞、肝细胞ABCA1和SR-BI的表达，提高3H]胆固醇醇转出率，并且上调血清炎性因子的水平，共同促进RCT，增加HDL功能，抑制AS进展。

Study on the effects of pioditazone on anti-atherosclerotic mechanism in atherosclerotic rabbits

Objective To analysis in atherosclerosis condition of peroxisome proliferator-activated receptor agonist （PPARγ） pioglitazone on liver cells, macrophage ATP binding cassette transporter AI, B type I scavenger receptor （Scavenger receptor, class B type I, SR-BI） 3H ] effect and cholesterol out rate, the plasma level of inflammatory factors in the process, reverse cholesterol transport, the HDL function will be heavily influenced by pioglitazone in a large extent, besides,it can also play a role to prevent atherosclerosis, this paper main- ly studies the mechanism is specific to its. Methods The experiment object is used in Janpanese white rabbits,the number is 30,which is divided into 3 experimental groups, respectively is normal and atherosclerotic, and pioglitazone group. For the first group, the food is or- dinary diet ; and the second group, the high fat food that is; third groups based on the second team, in the provision of pioglitazone, the specification is 5 mg/kg ~ d. After feeding for 20 days, blood lipids were measured in vivo, the main method is the enzymatic method;in addition to the determination of phagocytic cells, and liver surface ABCA1, SR-BI protein, methods for their use is the flow cytometry; not only that, but also to measure cholesterol transfer rate,the use of liquid scintillation counter; Determination of serum inflammatory factor concentration using ELISA. Results After 20 weeks, compared with the normal control group, AS group, ABCA1 and SR-B1 of liver cells and peritoneal macrophages 3 H ] cholesterol transfer rate was significantly reduced, the difference was statistically significant; com- pared with AS group, pioglitazone group of liver cells and peritoneal macrophages of ABCA1, SR-B1, 3H ] cholesterol transfer rate ex- pression increased significantly, the difference was statistically significant. Conclusion Pioglitazone can not only make the peritoneal macrophage was increased, but also can promote the expression of ABCA1 and SR-BI in liver cells, in addition, cholesterol transfer rate is improved, but also that of serum inflammatory factor level is imProved, and jointly promote the increase of RCT, HDL function, inhibition of AS progress.