高效毛细管电泳法同时测定丹参饮片中5种酚酸类成分

 目的 建立高效毛细管电泳法同时测定丹参饮片中迷迭香酸、原儿茶醛、丹参素、丹酚酸B、丹酚酸A含量的方法，同时考察丹参药材适宜干燥温度。方法 10 mmol·L^-1硼酸-12 mmol·L^-1磷酸二氢钠-10 mmol·L^-1SDS为电泳介质，未涂渍标准熔融石英毛细管(75 μm×64.5 cm，有效长度56 cm)为分离通道，分离电压为18 kV，检测波长为206 nm，毛细管温度为20 ℃，压力进样:5 kPa×6 s。结果 5种指标成分的浓度与峰面积的线性关系良好(r>0.999 0)；干燥温度以100 ℃为宜。结论 该方法简单、准确，重复性较好，可用于丹参饮片的质量评价和控制。

Simultaneous Determination of Five Phenolic Acids in Salviae Miltiorrhizae Radix et Rhizoma by HPCE

OBJECTIVE To establish an high performance capillary electrophoresis (HPCE) method for simultaneous determination of rosmarinic acid， protocatechualdehyde， danshensu， salvanic acid B， and salvianolic acid A in Salviae Miltiorrhizae Radix et Rhizoma. And to determine the optimum drying temperature for the herb. METHODS 10 mmol·L^-1 boric acid -12 mmol·L^-1 monosodium phosphate-10 mmol·L^-1 SDS was used as buffer solution， an uncoated fused silica capillary column (75 μm×64.5 cm， 56 cm of effective length) was used, the separation voltage was 18 kV， detection wavelength was 206 nm， column temperature was maintained at 20 ℃， and sample was injected at 5 kPa×6 s. RESULTS Five components showed good linearity (r>0.999 0) in the ranges of the tested concentrations. The optimum drying temperature was 100 ℃. CONCLUSION The method is simple， accurate and reproducible， and can be used for the quality control of Salviae Miltiorrhizae Radix et Rhizoma.