| 白英提取液对Hela细胞凋亡及Fas/FasL基因表达的影响 |
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| 引用本文： | 韦星,李朝敢,农嵩,朱晓莹,黄晓敏. 白英提取液对Hela细胞凋亡及Fas/FasL基因表达的影响[J]. 中药材, 2006, 29(11): 1203-1206 |
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| 作者姓名： | 韦星 李朝敢 农嵩 朱晓莹 黄晓敏 |
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| 作者单位： | 右江民族医学院生物化学与分子生物学教研室,广西百色,533000 |
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| 摘 要： | 目的:探讨白英提取液对Hela细胞凋亡及Fas/FasL基因表达的影响。方法:采用MTT法检测细胞增殖抑制率,流式细胞仪检测凋亡,二步法免疫组化检测Fas蛋白表达,半定量RT-PCR检测Fas和FasL mRNA表达。结果:白英提取液对Hela细胞增殖有较强的抑制作用,且这种作用呈一定的剂量-时间依赖性;细胞凋亡率明显升高;Fas基因mRNA和蛋白表达显著上升,FasL mRNA表达明显下降。结论:白英提取液通过上调Fas基因和下调FasL基因表达,诱导细胞凋亡,从而抑制Hela细胞增殖。
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| 关 键 词： | 白英 Hela细胞 细胞凋亡 |
| 文章编号： | 1001-4454(2006)11-1203-04 |
| 收稿时间： | 2006-02-28 |
| 修稿时间： | 2006-02-28 |
The Influence of Solanum lyratum Thunb Extract on Apoptosis and the Expression of Fas/FasL Genes in Hela Cells |
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| WEI Xing,LI Chao-gan,NONG Song,ZHU Xiao-ying,HUANG Xiao-min. The Influence of Solanum lyratum Thunb Extract on Apoptosis and the Expression of Fas/FasL Genes in Hela Cells[J]. Journal of Chinese medicinal materials, 2006, 29(11): 1203-1206 |
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| Authors: | WEI Xing LI Chao-gan NONG Song ZHU Xiao-ying HUANG Xiao-min |
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| Affiliation: | Department of Biochemistry and Molecular Biology, Youjiang Medical College for Nationalities, Baise 533000, China |
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| Abstract: | Objective: To explore the effects of Solanum lyratum Thunb(SL) extract on the apoptosis and the expression of fas and fasL genes in Hela cells.Methods: The proliferation inhibitory rate was evaluated by MTT assay.Induction of cell apoptosis rate was determined by flow cytometry.The expression of Fas protein was detected by two-step immunhistochemical staining.The expression of fas and fasL mRNA was detected by semi-quantitive RT-PCR.Results: SL extract displayed strong proliferation inhibitory effect in a dose-and-time-dependent manner against Hela cell.The rate of apoptosis was increased obviously.The expression of fas mRNA and protein was increased significantly,and fasL mRNA was decreased markedly.Conclusion: SL can induce apoptosis by up-regulating expression of fas and fasL genes,and inhibit the development of Hela cells. |
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| Keywords: | Fas FasL |
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