SATB1在不同侵袭潜能肝癌细胞株的表达

目的研究SATB1在不同侵袭潜能的人肝癌细胞株表达状况。方法分别用荧光定量PCR，RT-PCR，Western blotting及免
疫荧光方法，检测人永生化肝细胞株HL-7702及肝癌细胞株HepG2、SMMC-7721、MHCC97L、MHCC97H、HCCLM3 中SATB1
的表达。结果相对于HL-7702，SATB1 mRNA在其余5 种肝癌细胞株中都有较高程度的表达，其中高侵袭性HCCLM3、
MHCC97H 表达最高，MHCC97L 次之，SMMC-7721、HepG2 最低（P<0.001）；Western blotting 分析HepG2、SMMC-7721、
MHCC97L、MHCC97H、HCCLM3肝癌细胞株的蛋白相对表达量分别为0.271±0.002；0.351±0.023；0.621±0.026；0.878±0.026；
1.236±0.006，高侵袭性HCCLM3相当于HepG2的4.6倍（P<0.001）；免疫荧光显示SATB1在5种肝癌细胞株的胞浆和胞核内均
有分布，高侵袭性细胞株HCCLM3、MHCC97H表现强阳性染色。结论SATB1在不同侵袭潜能肝癌细胞株中的表达有差异，
与肝癌转移密切相关。

Expression of SATB1 in hepatocellular carcinoma cell lines with different invasive capacities

Objective To study the expression of special AT-rich sequence binding protein 1(SATB1) in hepatocellular carcinoma(HCC) cell lines with different invasive capacities.Methods SATB1 expression was detected using real-time fluorescence quantitative PCR,RT-PCR,Western blotting and immunofluorescence in immortalized liver cell line HL-7702,noninvasive HCC cell lines HepG2 and SMMC-7721,MHCC97L cells with low invasiveness,and highly invasive cell lines MHCC97H and HCCLM3.Results In comparison with HL-7702 cells,all the 5 HCC cell lines showed overexpression of SATB1 mRNA,which was the highest in the highly invasive HCCLM3 and MHCC97H cells,followed by MHCC97L cell line,and then by SMMC-7721 and HepG2 cell lines(P<0.001).The relative expression quantity of SATB1 protein in HepG2,SMMC-7721,MHCC97L,MHCC97H,and HCCLM3 cell lines was 0.271±0.002,0.351±0.023,0.621±0.026,0.878±0.026,and 1.236±0.006,respectively.SATB1 expression level in HCCLM3 cell line was 4.6-fold higher than that in HepG2 cell line(P<0.001).SATB1 was found to localize in the cytoplasm and cell nuclei of the 5 HCC cell lines,and the highly invasive HCCLM3 and MHCC97H cell lines showed a strong positive staining for SATB1 in immunofluorescence assay.Conclusion SATB1 expression levels differ distinctly between the HCC cell lines with different invasive capacities and are possibly associated with the metastatic potentials of the cells.