首页 | 本学科首页   官方微博 | 高级检索  
     


Platelet-derived growth factor receptor is expressed by cells in the early oligodendrocyte lineage
Authors:J. A. Ellison  J. de Vellis
Abstract:We report the loaclization of PDGFRα mRNA (PDGFRα) in phenotypically defined cells during the first postanatal week of rat forebrain development. Using a method of combined immunocytochemistry and in situ hybridization we have demonstrated the cellular colocalization of PDGFRα mRNA with GD3 ganglioside or 04 sulfatide, phenotypic markers of oligodendrocytes, in the gray and white matter of the dorsal cerebral cortex at all ages studies. Population analysis of the PDGFRα+/G3+ and PDGFRα+/analysis of the PDGFRα+/GD 3+and PDGFRα+/04+ cells revealed that three populations express PDGFRα: GD 3+, GD 3+/04+, and 04+, corresponding to two lineage stages, progenitor and preoligodendrocyte, in oligodendrocyte development. Immature oligodendrocytes, identifice by galactocerebroside immunoreactivity, did not express detectable levels of PDGFR α mRNA. Post-mitotic neurons, identifice by immunoperodxidase localization of the 68 kD neurofilament, and astrocytes identified by S-100 or GFAP immunoreactivity were also negative for PDGFRα mRNA occured in oligodendrocyte cell populations which are post-migratory and proliferative, but which do not express myelin proteins characteristic of post-mitotic oligodendrocytes. © 1994 Wiley-Liss, Inc.
Keywords:PDGF  glial pregenitor  04 sulfatide  development  cerebrum
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号