窄谱中波紫外线联合吡美莫司乳膏对成人型特应性皮炎患者PBMC产生eotaxin和CCR3表达的影响

 目的:检测窄谱中波紫外线（NB-UVB)联合吡美莫司乳膏治疗前后,特应性皮炎 (AD) 患者外周血嗜酸性粒细胞活化趋化因子(eotaxin)与其相应受体CCR3的表达,以探讨其治疗特应性皮炎的相关机制。方法: 采用窄谱中波紫外线联合吡美莫司乳膏治疗30例成人型AD患者,酶联免疫吸附试验检测治疗前后血清中eotaxin水平; 同时用流式细胞仪分析外周血中CCR3的表达。结果: 治疗前,AD患者血清eotaxin水平为(133.86±42.23) pg/mL,CCR3表达水平为(23.10±6.31)%;治疗后,AD患者血清eotaxin水平为(101.54±35.63) pg/mL,较治疗前明显降低(t=3.20,P＜0.01);外周血 CCR3表达水平为(16.52±6.59)%,较治疗前亦明显降低(t=3.59,P＜0.01)。结论: 窄谱中波紫外线联合吡美莫司乳膏可能通过降低eotaxin、CCR3表达,从而减少嗜酸性粒细胞的募集、活化,发挥其治疗特应性皮炎的作用。

Effects of NB-UVB with pimecrolimus cream on the expression of eotaxin in serum and CCR3 on peripheral blood monocytes in patients with adult atopic dermatitis

Objective:To compare expression levels of eotaxin in the serum and CCR3 on the peripheral blood monocytes(PBMC)prior to and after the treatment with NB-UVB plus pimecrolimus cream,in order to elucidate the pathogenesis of atopic dermatitis(AD).Methods:Serum level of eotaxin was detected by enzym-linked immunosorbent assay(ELISA)in 30 adult patients with AD prior to and after the treatment.CCR3 expression on the peripheral blood monocytes was analyzed by flow cytometry.Results:Treatment of AD with NB-UVB plus pimecrolimus cream markedly lowered the serum levels of eotaxin[(133.86±42.23)pg/mL vs.(101.54±35.63)pg/mL,P<0.01],and CCR3 on PBMC[(23.10±6.31)%vs.(16.52±6.59)%,P<0.01].Conclusions:Combination of NB-UVB with pimecrolimus cream could decrease the expression levels of eotaxin and CCR3,resulting in a reduction in recruitment and activation of eosinophils,consequently leading to improvement of AD.This study also suggested that combination of NB-UVB with pimecrolimus cream is safe and effective for AD.