高效液相色谱法测定育发凝胶中胸腺素β_4(17~23)片段的含量

目的建立育发凝胶中胸腺素β4(17~23)七肽片段的含量测定方法。方法采用反相C-12色谱柱,以三氟乙酸-乙腈-水为流动相进行梯度洗脱,检测波长为215 nm,用高效液相色谱法测定育发凝胶中胸腺素β4七肽片段的含量。结果胸腺素β4七肽片段含量的线性范围在0.02~0.18 mg/mL,r=0.999 8(n=5),平均回收率为100.06%,RSD为0.59%(n=9)。结论此法操作简便、快捷、准确、专属性高,可用于育发凝胶中胸腺素β4七肽片段的含量测定。

Determination of the content of thymosin β4 seven peptide fragment (17-23) in trichogeous gel by HPLC

Purpose To establish a method for the determination of the thymosin β_4 seven peptide fragment(17-23) in trichogeous gel.MethodsThe thymosin β_4 seven peptide in trichogeous gel was determined with reversed phase HPLC under the condition as follows: column: Synergy~(tm) RP-MAX(C-12,150 mm×4.6 mm,4 μm),mobile phase: trifluroacetic-acetonitrile-water,in gradient;detection wavelength:215 nm.ResultsA good linear relationship(r=0.999 8) was obtained within the range of 0.02-0.18 mg/mL with an average recovery of 100.06% and RSD of 0.59%(n=9).ConclusionThe established reversed phase HPLC method was simple,rapid and accurate for the content determination of the thymosin β_4 seven peptide fragment(17-23) in trichogeous gel.