野生型p53基因抑制胆囊癌细胞裸鼠体内生长的实验研究

目的：研究转染野生型p53（wtp53）基因对胆囊癌细胞裸鼠体内生长的影响。方法：在脂质体介导下将含有wtp53的真核表达质粒pCMV—p53，导入GBC—SD细胞中。用G418筛选，建立转染克隆细胞系。以PCR、RT—PCR和蛋白质印迹法证实外源p53基因的整合与表达。用裸鼠移植瘤试验检测体内致瘤性的影响。结果：野生型p53基因成功的导入胆囊癌细胞系，转染细胞中存在外源p53基因及蛋白的稳定表达。表达外源wtp53的GBC—SD—wtp53细胞，裸鼠体内致瘤性受到显著抑制。结论：野生型p53基因可有效抑制胆囊癌细胞在裸鼠体内的生长。

EXPERIMENTAL STUDY OF EXOGENOUS WILD- TYPE p53 GENE IN INHIBITING THE GROWTH OF GALLBLADDER CANCER CELLS IN VIVO

Objective:To study the effect of exogenous wild-type p53 gene on the growth of human gallbladder cancer cells in vivo in nude mice.Methods: Eukaryotic expressing plasmid pCMV-p53 was introduced into GBC-SD cell lines mediated by liposome.Growing transfected cells were selected by G418.The presence and expression of exogenous p53 gene was detected by PCR,RT-PCR and Western blotting,the xenograft in nude mice was performed to examine the effect of tumorigenicity.Results:The results of PCR,RT-PCR and Western blotting showed exogenous p53 gene had successfully transfected into GBC-SD cells and obtained high expression.The GBC-SD-wtp53 cell lines tumorigenicity were significantly inhibited after transfection of wtp53.Conclusion:The expression of exogenous wild-type p53 gene could effectively inhibit the growth of GBC-SD cell lines in vivo in nude mice.