| Effect of siRNA Targeting Survivin Gene on the Biological Behavior of Hepatocellular Carcinoma |
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| 引用本文： | 卢昕 郑启昌 熊俊. Effect of siRNA Targeting Survivin Gene on the Biological Behavior of Hepatocellular Carcinoma[J]. 华中科技大学学报(医学英德文版), 2005, 25(1): 48-50. DOI: 10.1007/BF02831385 |
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| 作者姓名： | 卢昕 郑启昌 熊俊 |
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| 作者单位： | DepartmentofHepatobiliarySurgery,UnionHospital,TongjiMedicalCollege,HuazhongUniversityofScienceandTechnology,Wuhan430022,China |
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| 基金项目： | This project was supported by a grant from National Na ture Sciences Foundation of China (No. 30271242) |
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| 摘 要： | To study the influence of siRNA targeting survivin gene on the biological behavior of hepatocellular carcinoma (HCC), one pair of 21bp reverse repeated motifs of survivin target sequence with 9 spacers were synthesized and inserted into plasmid psilencer2.1 to generate siRNA eukaryotic expression vector. After stable transfection into HepG2 cells, the biological behaviors of the survivin siRNA transfected HCC cells were observed. After the recombinant plasmid Psilence( )-survivin was successfully constructed, survivin mRNA and protein expression inhibition ratio reached 73% and 75% respectively compared to control groups. Transfected cells with survivin siRNA demonstrated significantly inhibited cell growth and increased apoptosis. Subsequent study in nude mouse model demonstrated lower succeeding rate in cells transfected with survivin siRNA and slow growth rate. The results elucidated the siRNA targeting survivin gene could specially suppress its expression in HepG2 cells and inhibit tumor cells growth both in vivo and in vitro. This provides a theoretical basis to turn the drug resistance in tumor cells.
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| 关 键 词： | siRNA 生物学行为 肝细胞癌 靶向生存基因 基因表达 |
| 收稿时间： | 2004-03-02 |
Effect of siRNA targeting survivin gene on the biological behavior of hepatocellular carcinoma |
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| Lu Xin,Zheng Qichang,Xiong Jun. Effect of siRNA targeting survivin gene on the biological behavior of hepatocellular carcinoma[J]. Journal of Huazhong University of Science and Technology. Medical sciences, 2005, 25(1): 48-50. DOI: 10.1007/BF02831385 |
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| Authors: | Lu Xin Zheng Qichang Xiong Jun |
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| Affiliation: | Department of Hepatobiliary Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China |
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| Abstract: | To study the influence of siRNA targeting survivin gene on the biological behavior of hepatocellular carcinoma (HCC), one pair of 21bp reverse repeated motifs of survivin target sequence with 9 spacers were synthesized and inserted into plasmid psilencer2.1 to generate siRNA eukaryotic expression vector. After stable transfection into HepG2 cells, the biological behaviors of the survivin siRNA transfected HCC cells were observed. After the recombinant plasmid Psilence(+)-survivin was successfully constructed, survivin mRNA and protein expression inhibition ratio reached 73 % and 75 % respectively compared to control groups. Transfected cells with survivin siRNA demonstrated significantly inhibited cell growth and increased apoptosis. Subsequent study in nude mouse model demonstrated lower succeeding rate in cells transfected with survivin siRNA and slow growth rate. The results elucidated the siRNA targeting survivin gene could specially suppress its expression in HepG2 cells and inhibit tumor cells growth both in vivo and in vitro. This provides a theoretical basis to turn the drug resistance in tumor cells. |
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| Keywords: | hepatocellular carcinoma siRNA survivin expression inhibition |
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