| 汉防己甲素脂质体包封率的测定 |
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| 引用本文： | 桂双英,温志强,彭代银,开伟华. 汉防己甲素脂质体包封率的测定[J]. 安徽中医学院学报, 2009, 28(5): 70-72 |
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| 作者姓名： | 桂双英 温志强 彭代银 开伟华 |
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| 作者单位： | 安徽中医学院药学院,安徽省"115"现代中药研发创新团队现代中药安徽省工程技术研究中心,安徽,合肥,230031 |
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| 基金项目： | 安徽省优秀青年科技基金项目 |
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| 摘 要： | 目的：建立汉防己甲素脂质体包封率的测定方法。方法：采用葡聚糖凝胶柱分离脂质体与游离药物，采用高效液相色谱法（high performance liquid chromatography，HPLC）测定脂质体中汉防己甲素的包封率。结果：葡聚糖凝胶G50可有效分离脂质体和游离药物；汉防己甲素在2～12μg／ml时与峰面积线性关系良好，y=8368x-897．33，r=0．9995。结论：HPLC法简单可行，结果准确，可用于测定汉防己甲素脂质体的包封率。
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| 关 键 词： | 汉防己甲素 脂质体 葡聚糖凝胶G50 高效液相色谱法 包封率 |
Determination of Entrapment Efficiency of Tetrandrine Liposomes |
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| GUI Shuang-ying,WEN Zhi-qiang,PENG Dai-yin,KAI Wei-hua. Determination of Entrapment Efficiency of Tetrandrine Liposomes[J]. Journal of Anhui Traditional Chinese Medical College, 2009, 28(5): 70-72 |
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| Authors: | GUI Shuang-ying WEN Zhi-qiang PENG Dai-yin KAI Wei-hua |
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| Affiliation: | (Department of Pharmacy, Anhui College of Traditional Chinese Medicine ＆ Anhui ＂115＂ Innovation Team for Research and Development of modern Chinese Materia ＆ Anhui Engineering Research Center for Modern Chinese Materia Medica , Anhui Hefei 230031, China) |
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| Abstract: | Objective： To establish a method for determining the entrapement efficiency of tetrandrine liposomes. Methods： The sephadex G-50 column was used for separating liposomes from free drugs, and then the entrapment efficiency of tetrandrine liposomes was determined by high performance liquid chroma- tography （HPLC）. Results..~ The tetrandrine liposomes could be effectively separated from free drugs using sephadex G-50. Tetrandrine showed a good linear relation with the peak area at a range of 2 - 12μg/ml, y=18 368 x=897.33,r=0. 999 5. Conclusion： HPLC method is simple and accurate, and it can be used for the determination of entrapement efficiency of tetrandrine liposomes. |
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| Keywords: | Tetrandrine Liposomes Sephadex G-50 High performance liquid chromatography Entrapement efficiency |
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