纤溶酶原激活剂及其抑制因子1在大鼠脊髓急性损伤时的表达

观察大鼠脊髓急性损伤时组织型纤溶酶原激活剂和纤溶酶原激活剂抑制因子１表达的变化，了解ｔ－ＰＡ、ＰＡＩ－１在脊髓急性损伤后所起的作用。方法３５只Ｗｉｓｔａｒ大鼠用改良Ａｌｌｅｎ法制成脊髓中度急性损伤模型伤后２，６，２４，７２，１６８，２４０小时取材分别进行组织学，免疫组化，图像分析。

Expression of Tissue-type Plasminogen Activator and Its Inhibitor-1 in Acute Rat Spinal Cord Injuries

Objective To observe the changes of tissue-type plasminogen activator (t-PA) and plasminogen activator inhibitor-1 (PAI-1) expression in acute rat spinal cord injuries (SCI), to learn about the effect of t-PA and PAI-1 on the process of acute spinal cord injury. Methods Using thirty-five Wistar rats, the moderately acute spinal cord injury models were made with modified Allien's methods. Histology examination, immunohistochemistry and map analyses were performed after 2, 6, 24, 72, 168, 240 hours of injury. Results After moderately acute spinal cord injury, pathology changes such as bleeding, thrombosis, liquescence, necrosis, etc occurred in the local area of injured spinal cord. Immunohistochemistry and map analysis demonstrated that the expression of t-PA reached the maximum in 3 days of injury, and subsequently reduced; but only a small amount of PAI-1 was expressed in 3 days of SCI, and then increased gradually. Conclusions t-PA and PAI-1 participate in the process of acute spinal cord injury. PAI-1 has some effects on protecting the remnant motor neurons and gliocytes.