再造组织工程化平滑肌组织的实验研究

目的采用组织工程技术，将培养扩增的膀胱平滑肌细胞与国产可降解的聚合聚乙交酯丙交酯（PLGA）支架材料复合构建并植入裸鼠体内进行培育，探讨再造组织工程化平滑肌组织的可行性。方法从幼兔取膀胱，机械分离与酶消化获取培养平滑肌细胞，传代扩增平滑肌细胞后将其接种到国产PLGA支架材料的表面作为实验组，未接种细胞的单纯支架材料作为对照组。分别将它们植入到裸鼠体内进行培育。经裸鼠体内培育4周、8周后取材并进行大体观察、组织学及免疫组织化学检测。结果实验组标本经HE和Masson染色显示在材料表面形成数层平滑肌细胞层，经抗平滑肌α-肌动蛋白免疫组织化学染色呈棕黄色阳性。随着培育时间的延长，支架材料降解明显，而平滑肌细胞层进一步增殖形成平滑肌组织。对照组经HE染色材料表面见有少量成纤维细胞沉积，Masson染色示兰色，经抗平滑肌α-肌动蛋白免疫组织化学染色为阴性。结论采用国产PLGA聚合物为支架材料可再造出组织工程化平滑肌组织，为多种含平滑肌组织空腔脏器的组织工程研究奠定基础。

Reconstruction of smooth muscles tissue with tissue-engineering technique

Objective This study evaluated the feasibility of reconstruction of smooth muscles tissue in vivo with tissue-engineering technique. Method The smooth muscles cells were obtained from young rabbits by mechanically and enzyme digested method. After expanded, the smooth muscle cells were seeded on the surface of a synthetic PLGA polymer and controlled with the polymer matrix without seeding cells. And then, the cells were implanted into subcutaneous pockets of athymic mice. The specimens were obtained and examined by gross in section 4 and 8 weeks later, histologically and immunohistochemically. Result Stained with HE and Masson, the polymer were covered by smooth muscles cells layers in experiment group, as polymer degradation, the smooth muscle cells layers increased markly. Immunocytochemical studies revealed that the cells were positively stained for anti-alpha smooth muscle actin. The surefaces of polymers were found fiber tissue deposition by HE and Masson staining and showed negative stains for anti-alpha smooth muscle actin by immunocytochemical staining in control group. The polymer covered with smooth muscle cells layers. Conclusion It is feasible to reconstruct bladder smooth muscles tissue with tissue-engineering technique.