沙丁胺醇对老年大鼠呼吸机相关肺损伤的干预作用

目的 探讨沙丁胺醇对老年大鼠呼吸机相关肺损伤（VILI）的干预作用.方法 将24只雄性Wistar老龄大鼠随机分为3组：正常对照组（第1组）、VILI未治疗组（第2组）、沙丁胺醇治疗组（第3组）,各接受不同潮气量的通气,通气时间为4h.检测各组大鼠左肺肺组织黄嘌呤氧化酶（XOD）与超氧物歧化酶（SOD）的活性,以及IL-2、IL-6、IL-8、TNF-α和丙二醛（MDA）的含量,同时观察肺组织HE染色和电镜下的变化.结果 第2组大鼠肺组织SOD与XOD的活性较第1组显著降低,而第3组较第2组明显提高（P均＜0.01）.第2组大鼠肺组织MDA、TNF-α、IL-2和IL-6含量较第1组升高（P均＜0.01）,第3组较第2组则显著降低（P均＜0.01）,各组大鼠肺组织IL-8含量差异无统计学意义（P＞0.05）;第2组大鼠肺组织HE染色显示炎症浸润明显,第3组较第2组明显减轻;电镜下第2组大鼠肺组织细胞及细胞器肿胀,毛细血管大量开放、充血,中性粒细胞激活,肺泡透明膜形成,第3组以上表现较第2组明显减轻.结论 沙丁胺醇能够减轻老年大鼠呼吸机相关肺损伤的炎症反应,提高了多种抗氧化物质的活性,改善微循环,从而减轻肺水肿的程度,对肺组织起到一定的保护作用.

Effects of Salbutamol on Ventilator-induced Lung Injury in Aged Rats

Objective To investigate the influence of salbutamol in aged rat model of ventilator induced lung injury （VILI）and its underlying mechanism. Methods Twenty-four healthy male Wistar rats were randomly divided into three groups tO receive different ventilation protocols for 4h： normal control group （group 1）, VILI without treatment （group 2）, VILI treated with salbutamol（group 3）. After ventilation, the activity of SOD, XOD and the level of MDA, TNF-a, IL-2, IL-6 and IL-8 in left lung tissue were detected. Lung histopathology was assessed by HE staining and electron microscopic observation. Results After 4h mechanical ventilation, the activity of SOD and XOD in lung tissue was significantly higher in group 3 than those of group 2. The level of MDA, TNF-a, IL-2 and IL-6 in lung tissue was significantly lower in group 3 than those of group 2. Histopathologic findings demonstrated that the destruction of lung tissue in group 2 was more serious than group 3. Conclusion Salbutamol reduced the inflammatory reaction, at the same time improved the various antioxidant activity and microcirculation, thereby reduced pulmonary edema of lung tissue and played certain protective function on ventilator-induced lung injury in aged rats.