蜂胶对幽门螺杆菌感染长爪沙鼠胃黏膜影响的初步观察

目的 观察蜂胶对幽门螺杆菌（Hp）感染长爪沙鼠胃黏膜的影响.方法 5周龄长爪沙鼠15只,随机分为对照组、感染组和蜂胶组.所有沙鼠禁食24h后,感染组和蜂胶组灌胃Hp菌液109cfu/ml,0.5 ml/只,连续灌胃3次,感染后4周每隔5d灌胃一次,感染后第16周连续5d每天灌胃一次加强感染;对照组灌胃无菌肉汤;蜂胶组在第22周和第24周灌胃蜂胶溶液,感染组和对照组灌胃生理盐水;27周处死所有试验动物,测量肝、肾、脾重量并计算脏器系数,测定血清IL-8,取胃黏膜进行Hp定量培养和病理学观察.结果 与对照组相比,感染组血清IL-8显著降低（P＜0.05）,与感染组相比,蜂胶组血清IL-8显著升高（P＜0.01）;各组肝脏、肾脏和脾脏脏器系数差异均不显著（P＞0.05）;蜂胶组的Hp数量（246±172 cfu/mg）显著低于感染组（1075±688 cfu/mg）（P＜0.05）;病理检查结果显示感染组胃黏膜炎症较严重.结论 蜂胶可抑制沙鼠胃内Hp的生长,具有一定的黏膜修复和器官保护作用.

Effects of Chinese Propolis on Gastric Mucosa of Mongolian Gerbils Infected with Helicobacter pylori

Objective To investigate the effects of Chinese propolis on gastric mucosa in Helicobacter pylori （Hp） infected Mongolian gerbils. Methods Fifteen 5-week-old Mongolian gerbils were randomly divided to three groups： control group, infection group, and propolis treatment group.The first two groups were inoculated with 0.5 ml of Hp （109 cfu/ml） by intragastric administration（i.g） for 3 times after 24 h fasting while control group was taken broth by i.g. 4 weeks after the first infection, i.g was repeated once every 5 days.At the 16th week, the gerbils were inoculated with 0.5 ml of Hp （109 cfu/ml） by i.g for consecutive 5 days to enhance infection. Gerbils in the propolis group were inoculated with propolis by i.g at the 22th and 24th week after the first infection, while the other two groups were inoculated with sterile saline in the same way. All the gerbils were sacrificed at the 27th week. The liver, kidney and spleen were removed to measure the organ coefficient. Gastric mucosa was removed to determine the concentration of Hp and for pathological observation. Results Compared with the control, the infection group exhibited a lower serum IL-8 level （P〈0.05）, while the propolis group significantly elevated the serum IL-8 level compared with the infection group （P〈0.01）; the organ coefficient of liver, kidney and spleen were not significant among groups （P〉0.05）; the concentration of Hp in the propolis group （2.46 ± 1.72 cfu/mg） was significantly lower （P〈0.05） than that in the infection group （10.75 ±6.88 x 10^2cfu/mg） （P〈0.05）; histological examination showed more serious mucosal inflam- mation in the the infection group. Conclusion The Propolis may inhibit the growth of Hp in the stomach of gerbils, and enhance anti-inflammatory ability in Hp-infected gerbils. Chinese propolis may play a role in mucosal repair and organ protection.