Helicobacter pylori isolates from ethnic minority patients in Guangxi:Resistance rates,mechanisms,and genotype |
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Authors: | Li-Juan Zhao Yan-Qiang Huang Bing-Pu Chen Xiao-Qiang Mo Zan-Song Huang Xiao-Feng Huang Lian-Deng Wei Hong-Yu Wei Yuan-Hong Chen Hua-Ying Tang Gan-Rong Huang Yan-Chun Qin Xiao-Hua Li Lu-Yao Wang |
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Affiliation: | Li-Juan Zhao, Yan-Qiang Huang, Bing-Pu Chen, Xiao-Qiang Mo, Zan-Song Huang, Xiao-Feng Huang, Lian-Deng Wei, Hong-Yu Wei, Yuan-Hong Chen, Hua-Ying Tang, Gan-Rong Huang, Yan-Chun Qin, Xiao-Hua Li, Lu-Yao Wang, Department of Medical Microbiology and Immunology, You Jiang Medical College for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China |
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Abstract: | AIM: To investigate the rate of Helicobacter pylori (H. pylori) resistance to clarithromycin among ethnic minority patients in Guangxi, explore the underlying mechanisms, and analyze factors influencing genotype distribution of H. pylori isolates.METHODS: H. pylori strains were isolated, cultured and subjected to drug sensitivity testing. The 23S rRNA gene of H. pylori isolates was amplified by PCR and analyzed by PCR-RFLP and direct sequencing to detect point mutations. REP-PCR was used for genotyping of H. pylori isolates, and NTsys_2 software was used for clustering analysis based on REP-PCR DNA fingerprints. Factors potentially influencing genotype distribution of H. pylori isolates were analyzed.RESULTS: The rate of clarithromycin resistance was 31.3%. A2143G and A2144G mutations were detected in the 23S rRNA gene of all clarithromycin-resistant H. pylori isolates. At a genetic distance of 78%, clarithromycin-resistant H. pylori isolates could be divided into six groups. Significant clustering was noted among H. pylori isolates from patients with peptic ulcer or gastritis.CONCLUSION: The rate of clarithromycin resistance is relatively high in ethnic minority patients in Guangxi. Main mechanisms of clarithromycin resistance are A2143G and A2144G mutations in the 23S rRNA gene. Clarithromycin-resistant H. pylori isolates can be divided into six groups based on REP-PCR DNA fingerprints. Several factors such as disease type may influence the genotype distribution of H. pylori isolates. |
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Keywords: | Helicobacter pylori Antibiotic resistance Mechanism Clarithromycin Genotype |
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