A new model for the study of neuropathic pain after brachial plexus injury |
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| Institution: | 1. Department of Hand Surgery, Huashan Hospital, Fudan University, 12 Wulumuqi Zhong Road, Shanghai 200040, China;2. Key Laboratory of Hand Reconstruction, Ministry of Health, Shanghai, China;3. Shanghai Key Laboratory of Peripheral Nerve and Microsurgery, Shanghai, China;1. University of Utah Department of Orthopaedic Surgery, Salt Lake City, UT, USA;2. University of Utah, Division of Epidemiology, Department of Internal Medicine, Salt Lake City, UT, USA;1. Department of Physiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran;2. Department of Clinical Biochemistry, School of Medicine, Tarbiat Modares University, Tehran, Iran;3. Protein Chemistry Lab, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran;1. Department of Orthopaedics, Academic Medical Centre, 1105 AZ, Amsterdam, The Netherlands;2. Department of Orthopaedics, Amphia Hospital, Breda, 4819 EV, The Netherlands;1. Department of Orthopedic, Beijing Army General Hospital, Beijing, 100700, China;2. Department of Physical Medicine and Rehabilitation, Division of Pain Medicine, Mayo Clinic, Rochester, MN, 55901, USA |
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| Abstract: | The study was to introduce a new and reliable behavioral model of upper trunk of brachial plexus avulsion for the study of persistent neuropathic pain. 60 rats were divided into three groups randomly: upper trunk of brachial plexus avulsion (UTBPA) group (20), global brachial plexus avulsion (GBPA) group (20), and sham- operated group (20). The animals were tested for behavioral responsiveness before surgeries and 3, 7, 14, 21, 28, 56, 84 days after surgeries. The injured level of spinal cord was resected and the sections were processed for GFAP (astrocyte) and Iba1 (microglia) immunohistochemistry 3 weeks after surgeries. The UTBPA group developed significant signs both of mechanical and cold hypersensitivity, which matched the immunohistochemistry result, as well as the nature of avulsion was close to the clinical type of injury, the UTBPA group could be used as a suitable and effective persistent neuropathic pain model following brachial plexus injury. |
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| Keywords: | Neuropathic pain Brachial plexus injury Mechanical Allodynia Cold allodynia Astrocyte Microglia |
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