Plasma cell and serum antibody responses to influenza vaccine in preterm and full-term infants |
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| Affiliation: | 1. Department of Pediatrics, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, NY 14642, USA;2. Departments of Microbiology & Immunology, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, NY 14642, USA;3. Department of Biostatistics, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, NY 14642, USA;4. Department of Medicine, Emory University School of Medicine, 1648 Pierce Drive NE, Atlanta, GA 30307, USA;1. Graded Specialist (Prosthodontics), Department of Dental Surgery, Armed Forces Medical College, Pune 40, India;2. Associate Professor, Department of Dental Surgery, Armed Forces Medical College, Pune-40, India;1. National Centre of Epidemiology, Institute of Health Carlos III, Spain;2. National Centre for Microbiology, National Influenza Reference Laboratory, WHO-National Influenza Centre, Institute of Health Carlos III, Spain;3. CIBER Epidemiología y Salud Pública (CIBERESP), Institute of Health Carlos III, Spain;4. Subdirección de Salud Pública y Adicciones de Bizkaia, Pais Vasco, Spain;5. Instituto de Salud Pública, Navarra Institute for Health Research (IdiSNA), Pamplona, Spain;6. Dirección General de Salud Pública, Consejería de Sanidad de Castilla y León, Valladolid, Spain;7. Servicio de Epidemiología y Prevención Sanitaria, Dirección General de Salud Pública y Consumo de La Rioja, La Rioja, Spain;8. Servicio de Epidemiología, Dirección General de Salut Pública, Mallorca, Baleares, Spain;9. Servicio de Epidemiología. DGSC, Consejería de Bienestar Social y Sanidad, Ciudad Autónoma de Melilla, Spain;1. Department of Comparative Medicine, University of Washington, Seattle, WA, United States;2. Department of Pediatrics, University of Washington, Seattle, WA, United States;1. Division of Hematology-Oncology, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI 48105, USA;2. University of Michigan Medical School, Ann Arbor, MI 48105, USA;3. Veterans Administration Ann Arbor Healthcare System, 2215 Fuller Road, Ann Arbor, MI 48105, USA |
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| Abstract: | BackgroundPreterm (PT) infants are at greater risk for severe influenza infection and experience decrements in long-term antibody responses to vaccines. This may related to defects in antibody secreting cell (ASC) generation.ObjectiveTo investigate the relationships among the frequencies of influenza-specific antibody secreting cells, ASC numbers and subsets, and antibody responses to influenza vaccines (IV) among PT and full-term (FT) infants.Design/methodsWe enrolled 11 former PT (≤32 weeks′ gestation, ≤1500 g′ birth weight) and 11 FT infants, 6–17 months of age, receiving their first influenza immunizations. Infants received two doses of inactivated trivalent (T)IV or quadrivalent (Q)IV during the 2012–2013 and 2013–2014 influenza seasons, respectively, at 0 and 28 days, and blood was drawn at 0, 10, 35, and 56 days and 9 months. Vaccine-specific antibody was measured by hemagglutination inhibition (HAI) at 0 and 56 days and 9 months, vaccine-specific ASC numbers by enzyme linked immunospot (ELISPOT) at 10 and 35 days, and ASC subsets by flow cytometry at 0, 10 and 35 days.ResultsPT infants had post-vaccine HAI titers to all 4 vaccine strains at least equal to FT infants at 56 days and 9 months after beginning immunization. Influenza-specific ASC ELISPOT responses at 35 days were higher among PT than FT infants (median 100 v. 30 per 106 PBMC, p = 0.04). ASC numbers at 35 days were positively correlated with serum HAI titers at 56 days (ρ = 0.50–0.80). There were no statistical differences between PT and FT infants in the frequency of five ASC subsets and no specific ASC subset correlated with durability of serum antibody titers.ConclusionsInfluenza-specific ASC numbers in both FT and PT infants correlated with peak antibody titers, but ASC subsets did not correlate with durability of antibody response. |
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| Keywords: | Influenza vaccine Premature infant Antibody-secreting cell Antibody titer Plasma cell |
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