Recombinant Newcastle disease virus expressing the infectious bronchitis virus S1 gene protects chickens against Newcastle disease virus and infectious bronchitis virus challenge |
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| Affiliation: | 1. Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150069, People’s Republic of China;2. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, People’s Republic of China;1. Department of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, KS, USA;2. Department of Virology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt;3. Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA;4. Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA;5. Innovation Team for Pathogen Ecology Research on Animal Influenza Virus, Department of Avian Infectious Disease, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China;6. Department of Medicine, Division of Infectious Diseases, Icahn School of Medicine at Mount Sinai, New York, NY, USA;1. Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, Guangzhou 510642, China;2. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China;3. Huizhou Entry-Exit Inspection and Quarantine Bureau, Huizhou 516001, China;1. Department of Avian Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China;2. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, PR China;1. Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, No. 12 East Wenhui Road, Yangzhou, Jiangsu 225009, PR China;2. Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, PR China;3. Jiangsu Key Lab of Zoonosis, Yangzhou 225009, PR China |
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| Abstract: | The recombinant LaSota strain expressing a chimeric IBV S1 gene (rLaSota-S1) was constructed with the S1 gene of the LX4 type IBV ck/CH/LDL/091022. The expression of the S1 protein was detected by an indirect immunofluorescence assay and Western blotting. The rLaSota-S1 strain was slightly attenuated, and its growth dynamics were similar to that of the parental LaSota strain. Vaccination of specific pathogen-free chickens with the rLaSota-S1 strain induced NDV hemagglutination inhibition antibodies, and it protected chickens from challenge with virulent NDV. In addition, vaccination with the rLaSota-S1 strain induced IBV-specific IgG antibodies and cellular immunity; however, a single vaccination provided partial protection with reduced virus shedding. Better protection efficiency was observed after a booster vaccination, which resulted in higher antibody titers, significantly fewer disease symptoms, and reduced virus replication and shedding. Our results suggest that the rLaSota-S1 strain is a bivalent vaccine candidate against both NDV and IBV. |
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| Keywords: | Bivalent vaccine candidate Infectious bronchitis virus LX4 genotype Newcastle disease virus Recombinant LaSota strain |
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