Glutathione is a highly efficient thermostabilizer of poliovirus Sabin strains |
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| Affiliation: | 1. BIT Medical Convergence Graduate Program and Department of Microbiology and Immunology, School of Medicine, Kangwon National University, Chuncheon, Gangwon-do 200-701, Republic of Korea;2. Department of Biochemistry, College of Natural Sciences, Kangwon National University, Chuncheon, Gangwon-do 200-701, Republic of Korea;3. Department of Molecular and Cellular Biochemistry, School of Medicine, Kangwon National University, Chuncheon, Gangwon-do 200-701, Republic of Korea;1. Department of Microbiology and Immunology, School of Medicine, Southeast University, Nanjing, Jiangsu, PR China;2. Department of Microbiology and Immunology, School of Medicine, Shaoxing University, Shaoxing, Zhejiang, PR China;3. Department of Dermatology, Affiliated Zhongda Hospital, School of Medicine, Southeast University, Nanjing, Jiangsu, PR China;4. Division of Viral Hepatitis, Centers for Disease Control and Prevention, Atlanta, GA, USA;1. Departamento de Medicina Preventiva, Salud Pública y Microbiología, Facultad de Medicina, Universidad Autónoma de Madrid, Madrid, Spain;2. Departamento de Microbiología, Fundación Jiménez Díaz-UTE, Madrid, Spain;3. Departamento de Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Autovía A6, Km 7.5, 28040 Madrid, Spain;4. Alternative Gene Expression S.L. (ALGENEX), Centro empresarial, Parque Científico y Tecnológico de la Universidad Politécnica de Madrid, Campus de Montegancedo, 28223 Pozuelo de Alarcón, Madrid, Spain;1. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA), Talcahuano 1660, CP 1640 Martínez, Argentina;2. Centro de Virología Animal, Instituto de Ciencia y Tecnología Dr. César Milstein, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Saladillo 2468, CP 1440 Buenos Aires, Argentina;3. Biogénesis Bagó S.A., Ruta Panamericana Km 38.2, CP 1619 Garín, Argentina;1. College of Life and Environmental Sciences, Hangzhou Normal University, 310016 Hangzhou, Zhejiang, China;2. Date Palm Research Center, King Faisal University, P.O. Box 420, Al-hasa 31982, Saudi Arabia;3. Department of Food Science, Anqing Vocational & Technical College, 246003 Anqing, Anhui, China |
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| Abstract: | Glutathione (GSH) is the most abundant thiol peptide in animal cells and has a critical role in antioxidation. GSH was reported to be essential for stabilization of some enteroviruses, including poliovirus (PV), during viral morphogenesis. Here, we explored the potential use of GSH as a thermostabilizer of oral poliomyelitis vaccine (OPV) formulations. GSH significantly protected the three types of PV from heat-inactivation in a concentration-dependent manner. At a GSH concentration of 20 mM, nearly complete protection was observed against heating temperatures up to 53 °C for 2 min. GSH also markedly protected PV1 from heat-inactivation and this up to 6 h at temperatures of 44 °C and 46 °C and 3 h at 48 °C. The fact that GSH is naturally present at high concentration in the human body makes it an efficient candidate stabilizer for OPV formulations. |
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| Keywords: | Glutathione Poliomyelitis Cold chain Vaccine Sabin |
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