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Development and Evaluation of a Loop-mediated Isothermal Amplification Combined with Au-nanoprobe Assay for Rapid Detection of Mycobacterium tuberculosis |
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| Affiliation: | 4. Innovative Learning Center, Srinakharinwirot University, Bangkok 10110, Thailand;1. Department of Clinical Pathology, Faculty of Medicine, Vajira Hospital, Navamindradhiraj University, Bangkok 10110, Thailand;2. Departments of Biochemistry, Bangkok 10110, Thailand;3. Departments of Pathology, Faculty of Medicine, Srinakharinwirot University, Bangkok 10110, Thailand |
| Abstract: | Loop-mediated isothermal amplification (LAMP) has been proposed as an inexpensive and easy to perform assay for molecular diagnostics. We present a novel strategy for the detection of LAMP amplicons derived from Mycobacterium tuberculosis by the use of Au-nanoprobes. When applied to a total of 93 clinical specimens, the LAMP assay demonstrated sensitivity and specificity higher than that of polymerase chain reaction and culture. The Au-nanoprobe augmented LAMP test platform with its advantages of robust reagents and a simple colorimetric detection method can be adapted easily for the rapid detection of other infectious disease agents at a low cost. |
| Keywords: | Au-nanoprobe diagnosis loop-mediated isothermal amplification tuberculosis |
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