Salmonella Enteritidis with double deletion in phoP fliC and a competitive exclusion culture elicit substantial additive protective effects against Salmonella exposure in newly hatched chicks |
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| Institution: | 1. Institute of Bacterial Infections and Zoonoses at the Friedrich-Loeffler-Institute, Federal Research Institute for Animal Health, Naumburger Str. 96a, D-07743 Jena, Germany;2. Institute of Molecular Pathogenesis at the Friedrich-Loeffler-Institute, Federal Research Institute for Animal Health, Naumburger Str. 96a, D-07743 Jena, Germany;1. Department of Epidemiology, School of Public Health, University of Michigan, Ann Arbor, MI 48109, USA;2. Laboratorio Nacional de Virologia, Centro Nacional de Diagnóstico y Referencia, Ministry of Health, Managua, Nicaragua;3. Centro de Salud Sócrates Flores Vivas, Ministry of Health, Managua, Nicaragua;4. Sustainable Sciences Institute, Managua, Nicaragua;5. Division of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, CA 94720, USA;1. Department of Microbiology, University of Washington, Seattle, WA, USA;2. W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD, USA;3. Department of Internal Medicine, University of Rochester, School of Medicine and Dentistry, Rochester, New York, USA;4. Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins University, School of Medicine, Baltimore, MD, USA;5. Washington National Primate Research Center, University of Washington, Seattle, WA, USA;6. Department of Environmental Health Sciences, Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD, USA;1. Robert D. and Patricia E. Center for the Science of Health Care Delivery, Department of Health Sciences Research, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA;2. Department of Health Sciences Research, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA;3. Robert D. and Patricia E. Center for the Science of Health Care Delivery, Departments of Health Sciences Research and Pediatric and Adolescent Medicine, Mayo Clinic, 200 First Street SW, Rochester, MN, USA;4. Division of Health Policy and Management, School of Public Health, University of Minnesota, Mayo Building A302, 420 Delaware Street SE, Minneapolis, MN 55455, USA;5. Department of Health Outcomes and Behavior, H. Lee Moffitt Cancer Center and Research Institute, 12902 USF Magnolia Drive, Tampa, FL 33612, USA;6. Department of Family Medicine, Mayo Clinic, 200 First Street SW, Rochester, MN, USA;6. Hôpital Avicenne, Service des maladies infectieuses et tropicales, 93000 Bobigny, France;1. Institut Pasteur, Centre médical, Paris, France;7. Hôpital Bicêtre, Service de Parasitologie-Médecine tropicale, 94270 Le Kremlin-Bicêtre, France;8. Centre de Vaccinations internationales Air-France, 75010 Paris, France;9. Hôpital Robert Debré, Centre de Vaccinations internationales, 75019 Paris, France;10. HIA Bégin, Service des maladies infectieuses et tropicales, 94160 Saint-Mandé, France;11. Hôpital Louis Mourier, Centre de Vaccinations internationales, 92700 Colombes, France;1. Institut Pasteur, Centre médical, Paris, France;2. Institut Pasteur, Unité de Recherche et d''Expertise Immunité anti-virale, Biothérapie et Vaccins, Paris, France;3. Institut Pasteur, Unité de Recherche et d''Expertise Epidémiologie des Maladies Emergentes, Paris, France;4. Université de La Réunion et UM 134 PIMIT, plateforme CYROI, Sainte-Clotilde, France;5. Institut Pasteur de Dakar, Unité d’épidémiologie des Maladies Infectieuses, Senegal;1. Centre National de Recherche et de Formation sur le Paludisme (CNRFP), Ouagadougou, Burkina Faso;5. INSERM, Institut de Santé Publique, Pôle de Recherche Clinique, Paris, France;10. Department of Parasitology, Biomedical Primate Research Centre, Rijswijk, The Netherlands;6. AP HP, Hôpital Cochin, Plateforme d’immuno-monitoring vaccinal, Laboratoire d’Immunologie, Paris, France;7. INSERM U970, Paris, France;8. AP-HP, Hôpital Européen Georges Pompidou, Service d’Immunologie Biologique, Paris, France;1. Centre National de Recherche et de Formation sur le Paludisme (CNRFP), Ouagadougou, Burkina Faso;2. INSERM SC10-US19, Villejuif, France;3. INSERM CIC 1417, F-CRIN, I-REIVAC, Paris, France;4. Assistance Publique –Hôpitaux de Paris (AP HP), Hôpital Cochin, CIC Cochin-Pasteur, Paris, France;9. European Vaccine Initiative (EVI), Heidelberg, Germany;6. AP HP, Hôpital Cochin, Plateforme d’immuno-monitoring vaccinal, Laboratoire d’Immunologie, Paris, France;7. INSERM U970, Paris, France;8. AP-HP, Hôpital Européen Georges Pompidou, Service d’Immunologie Biologique, Paris, France;10. Department of Parasitology, Biomedical Primate Research Centre, Rijswijk, The Netherlands;11. Université Paris Descartes; Sorbonne Paris-Cité, Paris, France;1. Department of Microbiology and Immunology, Wake Forest School of Medicine, Winston-Salem, NC, United States;2. Department of Biostatistical Sciences, Wake Forest School of Medicine, Winston-Salem, NC, United States;3. Animal Resources Program, Wake Forest School of Medicine, Winston-Salem, NC, United States;4. Department of Pathology, Section of Comparative Medicine, Wake Forest School of Medicine, Winston-Salem, NC, United States;5. Department of Chemistry, Wake Forest University, United States |
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| Abstract: | A live Salmonella Enteritidis vaccine (SE 147N ΔphoP fliC), able to express both a homologous intestinal colonisation-inhibition effect and a systemic invasion-inhibition effect, was tested for its potential to generate a postulated additive protective effect in case of combined application with a competitive exclusion (CE) culture against Salmonella exposure in very young chicks. Both, SE 147N ΔphoP fliC and the CE culture alone were highly protective against systemic and intestinal colonisation of the challenge strain in case of moderate Salmonella exposure, consequently, additive protective effects in combined use could not be detected. However, in case of high Salmonella Enteritidis challenge with 106 cfu/bird at day 3 of life the combination of the ΔphoP fliC vaccine and the CE culture resulted in a protective effect much more pronounced than either of the single preparations and most substantial compared to untreated control birds. The term additive protective effects reflects the recognition that exclusion effects by gut flora cultures and inhibition effects by Salmonella vaccines are caused by different mechanisms. |
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| Keywords: | Chicken Competitive exclusion Inhibition Immune response |
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