Effect of infectious bursal disease (IBD) vaccine on Salmonella Enteritidis infected chickens |
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| Institution: | 1. Department of Poultry Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt;2. Department of Pathology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt;3. Department of Pharmacology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt;1. National Immunization Programme, Chinese Center for Disease Control and Prevention, China;2. Department of Epidemiology, School of Health Sciences, FIN-33014 University of Tampere, Finland;3. National Institute for Health and Welfare THL, Helsinki, Finland;1. Department of Clinical Sciences Lund, Section of Rheumatology, Lund University, Skåne University Hospital, SE-221 85 Lund, Sweden;2. Department of Infectious Diseases, Central Hospital Kristianstad, J A Hedlunds väg 5, SE-291 85 Kristianstad, Sweden;3. Department of Clinical Sciences Lund, Section of Infectious Diseases, Lund University, Skåne University Hospital, SE-221 85 Lund, Sweden;4. Department of Laboratory Medicine, Section of Microbiology, Immunology and Glycobiology, Lund University, Lund, and Clinical Immunology and Transfusion Medicine, Region Skåne, Sweden;1. Wayne State University School of Medicine, United States;2. Yale School of Medicine, United States;1. Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran;2. Food Microbiology Research Centre, Tehran University of Medical Sciences, Tehran, Iran;3. Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran;4. Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran;5. Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran;6. Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran;1. National Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Giza, 12618, Egypt;2. Zoonosis Science Center, Department of Medical Biochemistry and Microbiology, Uppsala University, 751 21, Uppsala, Sweden |
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| Abstract: | BackgroundChickens infected with both infectious bursal disease virus (IBDV) and Salmonella had higher mortality. In this work, we investigated the effect of IBDV vaccine (modified live-virus bursal disease vaccine, Nobilis strain 228E®) on experimentally infected chickens with Salmonella Enteritidis (SE).MethodsFour experimental groups were included in this study, negative control group, 228E®group, 228E® + SE infected group, and SE infected group. Chickens were ocularly administrated 228E® at 12 days of age and orally infected with S. Enteritidis at 13 days of age. Sera, intestinal fluid, blood, cloacal swabs and tissue samples were collected at 1, 2 and 3 weeks post vaccination (PV).ResultsThe recorded mortalities were higher in the 228E® + SE infected group, compared to the SE infected group. The anti-S. Enteritidis serum antibody titer and the intestinal mucosal IgA level were higher in the SE infected group at 2 and 3 weeks PV, compared to 228E® + SE infected group. S. Enteritidis fecal shedding and organ colonization were significantly higher in the 228E® + SE infected group than the SE infected group at 2 and 3 weeks PV. The 228E® + SE group had significantly lower bursa to body weight ratios at 2 and 3 weeks PV, as well as had higher bursal lesion scores than the SE infected group. IBDV vaccine depressed the specific-SE systemic and mucosal antibody responses, but did not affect the specific-SE cellular immune responses.ConclusionChickens administrated IBDV vaccine, followed by S. Enteritidis infection, could cause a significant effect on the bursa of Fabricius, resulting in failure of systemic and mucosal antibody responses to the S. Enteritidis and reduce the elimination and the clearance of S. Enteritidis. |
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| Keywords: | Chickens Infectious bursal disease virus vaccine |
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