Efficacy of a high potency O1 Manisa foot-and-mouth disease vaccine in cattle against heterologous challenge with a field virus from the O/ME-SA/Ind-2001 lineage collected in North Africa |
| |
| Affiliation: | 1. The Pirbright Institute, Ash Road, Pirbright, Surrey GU24 0NF, United Kingdom;2. Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna (IZSLER), Via Bianchi, 9, 25024 Brescia, Italy;3. Wageningen Bioveterinary Research, Laboratory Vesicular Diseases, Department of Virology, Houtribweg 39, 8221RA Lelystad, The Netherlands;1. Department of Veterinary Medicine, Infectious Diseases Division, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt;2. Ismailia, Egypt;3. Mansoura Provincial Laboratory, Animal Health Research Institute, Mansoura, Egypt;4. Department of Virology, Faculty Veterinary Medicine, Zagazig University, Zagazig, Egypt;5. Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, Gainesville, FL 32610, USA;6. Veterinary Diagnostic and Investigational Laboratory, College of Veterinary Medicine, University of Georgia, 43 Brighton Road, Tifton, GA, USA;1. The Pirbright Institute, Ash Road, Woking GU24 0NF, Surrey, UK;2. Foot-and-Mouth Disease Virus Laboratory, Research and Development Centre, Indian Immunologicals Limited, Gachibowli, Hyderabad 500 032, India;3. Project Directorate on Foot-and-Mouth Disease, IVRI Campus, Mukteswar-Kumaon, Nainital 263138, Uttarakhand, India;1. The Pirbright Institute, Ash Road, Pirbright, Surrey GU24 ONF, UK;2. National Institute for Animal Biotechnology, Hyderabad 500049, India;1. Animal and Plant Quarantine Agency, 175 Anyang-ro, Manangu, Anyang city, Gyeonggido, 430-757, Republic of Korea;2. Gyeonggi Province Veterinary Service Center, Anseong-si, Gyeonggi-do, 456-823, Republic of Korea;3. Veterinary College, Chungnam National University, Yuseonggu, Daejeon, 305-764, Republic of Korea;1. Transboundary Animal Diseases Programme, Onderstepoort Veterinary Institute, Agricultural Research Council, Private Bag X05, Onderstepoort 0110, South Africa;2. Department of Veterinary Tropical Diseases, Faculty of Veterinary Science University of Pretoria, Private Bag X04, Onderstepoort, 0110, South Africa;3. Department of Microbiology and Plant Pathology, Faculty of Agricultural and Natural Sciences, University of Pretoria, Pretoria 0002, South Africa;4. INTA, Instituto de Virología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas, Buenos Aires, Argentina |
| |
| Abstract: | Outbreaks of foot-and-mouth disease (FMD) in North Africa (2013) and the Gulf States (2013) of the Middle East have been caused by a FMD viral lineage (O/ME-SA/Ind-2001) that was before 2013 restricted to the Indian Sub-continent. This study was undertaken to assess the in vivo efficacy of a FMD virus emergency vaccine type O1 Manisa against heterologous challenge with a representative field virus (O/ALG/3/2014) from this emerging lineage. This widely available vaccine was selected since in vitro vaccine-matching results gave inconclusive results as to whether or not it would be protective. Three groups of five cattle were vaccinated with O1 Manisa (homologous potency ≥6PD50/dose) using study guidelines outlined in the European Pharmacopeia, and challenged at 21 days post-vaccination by tongue inoculation. All animals that were vaccinated with the lowest dose (1/16) of vaccine developed generalised FMD, defined as vesicular lesions at the feet. One animal vaccinated with a 1/4 dose of the vaccine also developed generalised disease, as did two animals vaccinated with the full dose of vaccine. These results indicate that the heterologous potency of this high potency O1 Manisa vaccine was approximately 3.5 PD50/dose. These data support the use of the O1 Manisa vaccine for FMD control in areas where FMDV is endemic e.g. North Africa, and motivate further studies to evaluate other vaccine candidates (or multivalent combinations) that might be potentially used for emergency purposes in FMD-free settings. |
| |
| Keywords: | Foot-and-mouth disease virus Vaccine In vivo potency Challenge |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
